混合型抗体EZH2/34βE12/p63在前列腺穿刺标本中的诊断意义  

Significance of detection of mixed type antibody EZH2/34βE12/p63 in needle biopsy specimen of prostatic lesions

作　　者：李江[1] 邱雁[1] 邱梁[1] LI Jiang QIU Yah QIU Liang(Department of Pathology, Jiangsu Provincial Geriat- rics Hospital, Nanjing 210024, China)

机构地区：[1]江苏省老年医院病理科,江苏省南京市210024

出　　处：《实用老年医学》2017年第5期458-460,共3页Practical Geriatrics

摘　　要：目的探讨混合型抗体EZH2/34βE12/p63在不同前列腺病变穿刺标本中的表达及其意义。方法在一张切片上采用免疫组织化学方法同时检测EZH2、34βE12、p63在前列腺癌(PCa)、良性前列腺增生(BPH)、高度上皮内瘤变(HGPIN)、非典型腺瘤样增生(AAH)中的表达。结果 52例PCa标本中,EZH2主要表达于前列腺腺上皮细胞核,阳性率为98.1%。60例BPH、10例HGPIN和14例AAH标本中,34βE12主要表达于前列腺基底细胞胞质,在上述病变中的阳性率分别为96.7%、100.0%和85.7%;p63主要表达于前列腺基底细胞胞核,在上述病变中的阳性率分别为98.3%、90.0%和92.9%。EZH2、34βE12和p63在PCa中表达与BPH、HGPIN、AAH中两两比较差异均有统计学意义(P<0.05)。结论联合检测混合型抗体EZH2/34βE12/p63 3种免疫学指标,在前列腺穿刺标本中对PCa与其他病变的鉴别诊断具有一定参考价值。Objective To study the expression and significance of EZH2/34βE12/p63 in needle biopsy specimen of prostatic lesions. Methods Expression of EZH2,34βE12 and p63 were detected using immunohistochemical method in the biopsy specimen of 53 cases of prostate cancer（ PCa）,60 cases of benign prostatic hyperplasia（ BPH）,10 cases of prostatic high grade intraepithelial neoplasia（ HGPIN） and 14 cases of prostatic atypical adenomotous hyperplasia（ AAH） with mixed type antibody in a single paraffin section. Results EZH2 was mainly expressed in the prostatic epithelia nuclear and the positive rate was 98. 1% in 52 cases of PCa. 34βE12 was mainly expressed in the prostatic basal plasma and the positive rate in 60 cases of BPH,10 cases of HGPIN and 14 cases of AAH was 96. 7%,100% and 85. 7% respectively.P63 was mainly expressed in the prostatic basal nuclear and the positive rate in BPH,HGPIN and AAH was 98. 3%,90. 0% and 92. 9% respectively. There were significant differences in expressions of EZH2,34βE12 and p63 among PCa,BPH,HGPIN and AAH（ P〈 0. 05）.Conclusions Immunohistochemical detection of EZH2,34βE12 and p63 in single paraffin sections could improve diagnostic and differential diagnostic accuracy in needle biopsy specimen of prostate lesion.

关 键 词：前列腺癌 EZH2 34ΒE12 P63 

分 类 号：R446.8[医药卫生—诊断学]