有氧运动与膳食干预对IR大鼠胰岛β细胞LKB1/AMPK信号的影响  

Effects of Aerobic Exercise and Diet Intervention on LKB1/AMPK Signal in Islet β Cell in IR Rats

作　　者：王孝强[1] 李荀[2] 曾凡星[1] 

机构地区：[1]北京体育大学运动人体科学学院,北京100084 [2]山东体育学院体育科学研究院,山东济南250102

出　　处：《沈阳体育学院学报》2017年第2期72-78,共7页Journal of Shenyang Sport University

基　　金：中央高校基本科研业务费专项资金资助课题(2015SYS013;2016BS024)

摘　　要：目的:探讨有氧运动与膳食干预对胰岛素抵抗(IR)大鼠胰岛β细胞LKB1/AMPK信号的影响,进一步阐明有氧运动与膳食干预改善IR的中心机制。方法:采用10w高脂膳食喂养的方法建立IR大鼠模型,建模成功后随机分为高脂膳食组(IRHC)、高脂膳食运动组(IRHE)、普通膳食组(IRNC)、普通膳食运动组(IRNE);正常大鼠随机分为安静对照组(NC)和单纯运动组(NE)。运动组大鼠进行中等强度有氧运动,跑台速度为20 m/min,坡度0%,60min/d,5 d/w,共计8 w。末次运动后24 h,禁食12 h后麻醉处死并取材。测定空腹血糖(FBG)、空腹胰岛素(FINS)、C肽、肿瘤抑制因子(LKB1)、腺苷蛋白激酶(AMPKα)蛋白和磷酸化表达。结果:1)10 w高脂膳食喂养后大鼠与普通膳食组相比体重、FBG、FINS、Homa-IR均显著升高(均P<0.01),Homa-ISI显著下降(P<0.01)。2)有氧运动与膳食干预8 w后对IR大鼠的影响:与NC组相比,NE组大鼠体重、FBG、C肽显著降低(P<0.05-0.01),胰岛β细胞pAMPKαThr172表达显著升高(P<0.05)。与IRHC组相比,IRHE组和IRNE组大鼠体重、FBG、FINS、C肽、Homa-IR均显著降低(P<0.05-0.01)、Homa-ISI均显著升高(P<0.05-0.01),IRNC组大鼠体重、C肽均显著降低(P<0.01)。与IRHC组相比,IRNC组和IRNE组大鼠胰岛β细胞LKB1、AMPKα、p-AMPKαThr172蛋白表达均显著升高(P<0.05-0.01)。结论:长期高脂膳食可降低胰岛β细胞LKB1/AMPK信号蛋白表达,促进胰岛素分泌;8 w膳食干预可增加IR大鼠胰岛β细胞LKB1/AMPK信号蛋白表达,减少胰岛素分泌。Objective：To investigate the impact of aerobic exercise and diet intervention on LKB 1/AMPK Signal in the islet β cell of insulin resistance rats. Methods：High-fat diet-induced insulin resistance rats were randomly divided into high-fat diet sedentary control group （ IRHC ） , high-fat diet exercise group （IRHE） and normal diet sedentary group （ IRNC）, normal diet exercise group（ IRNE）. The normal rats were randomly divided into sedentary control group （NC）and exercise group （NE）. Rats in all exercise groups underwent a moderate intensity treadmill （ 20m/min,0 % ,60 min/d, 5 d/w ） for 8 weeks. The blood in abdominal aorta and islet were collected after 12h fasting, FBG, FINS, C-peptide, LKB1, AMPKα and p- AMPKαThr172 were measured. Results： 1. Compared with the control group, the body weight, FPG level, FINS and HO- MA-IR of IRHE significantly increased after 10 weeks （ all P 〈 0. 01 ）, Homa-ISI significantly decreased （ P 〈 0. 01 ）. 2. The effect of aerobic exercise and diet changed. Compared with NC, the body weight, FBG and C-peptide of rats in NE signifi- cantly decreased （P 〈 0. 05 -0.01 ） and the protein expression of p-AMPKαThr172 in islet β cell significantly increased （P 〈 0. 05 ）. Compared with IRHC, the body weight, FBG, FINS, C-peptide and Homa-IR （P 〈 0. 05 -0.01 ） of rats in IRHE and IRNE significantly decreased and Homa-ISI significantly increased（ P 〈 0. 05 -0.01 ） ;The body weight and C-peptide significantly decreased（ P 〈 0. 01 ）. The protein expression of LKBl, AMPKct and p-AMPKαThr172 （ P 〈 0. 05 - 0.01 ） in islet β cell of rats in IRNC and IRNE significantly increased. Conclusions ： Long-term high-fat diet can reduce the expres- sion of LKB1/AMPK in pancreaticβ-cell ,leading to promote insulin secretion. The restoration of normal diet can increase the expression of LKB1/AMPK in pancreatic β-cell ,decrease insulin secretion.

关 键 词：有氧运动 膳食干预 胰岛素抵抗 胰岛β细胞 LKB1/AMPK 

分 类 号：G804.7[文化科学—运动人体科学]