在体单向肠灌流模型研究白芷中三种成分的吸收特性  

Intestinal Absorption of Three Components in Angelicae Dahuricae by in situ Single Pass Intestinal Perfusion Model in Rat

作　　者：闫涛[1] 王琴会[1] 姜维[1] 陈曦[1] 赵美[1] 刘新友[1] 

机构地区：[1]第四军医大学唐都医院药剂科,陕西西安710038

出　　处：《现代生物医学进展》2017年第17期3238-3242,共5页Progress in Modern Biomedicine

基　　金：陕西省中医药管理局资助项目(13-ZY039)

摘　　要：目的:研究白芷中有效成分欧前胡素、异欧前胡素和花椒毒酚在大鼠小肠各段的吸收特征。方法:采用大鼠在体单向肠灌流模型结合HPLC法同时测定肠灌流液中欧前胡素、异欧前胡素和花椒毒酚含量的变化,考察高、中、低三组剂量下三种成分在十二指肠、空肠和回肠的吸收特性。结果:三种成分随着剂量的增大,吸收速率常数(Ka)和有效渗透系数(Peff)逐渐增大。三种成分相同剂量下各肠段间吸收均无显著性差异,其吸收大小顺序为:欧前胡素>异欧前胡素>花椒毒酚。结论:白芷三种主要成分在各肠段均吸收良好,吸收大小呈剂量相关性。Objective： To investigate absorption mechanism of imperatorin, isoimperatorin and methoxsalen in radix of Angelicae dahurieae. Methods： In situ single pass intestinal perfusion model was established, HPLC was used to determine the change of the concentration of imperatorin, isoimperatorin and xanthotoxol in different segments. The absorption parameters of these three analytes in dif- ferent intestinal segments were investigated. Results： With the increasing of doses for these three analytes, the absorption rate constant （Ka） and effective permeability coefficient （Peff） were increased. There were no significant differences in different segments of intestine in rat. The absorption order was imperatorin, isoimperatorin and xanthotoxol. Conclusion： Radix of Angelicae dahuricae was easily absorbed in the intestine, the degree of absorption was dose-dependent.

关 键 词：白芷 在体单向肠灌流 高效液相色谱 肠吸收 

分 类 号：Q95-3[生物学—动物学] R285.5[医药卫生—中药学]