紫色甘薯醇提物抗疲劳机制研究  

Study on anti-fatigue mechanism of alcohol extracts of purple sweet potato

作　　者：熊斌[1] 王杨科[1] 尚书凤[1] 路宏朝[1] 颜文博[1] 王琦[1] 

机构地区：[1]陕西省资源生物重点实验室陕西理工学院,汉中723000

出　　处：《中国食品添加剂》2017年第1期65-70,共6页China Food Additives

基　　金：陕西省重点实验项目(13JS022);陕西理工学院研究生创新基金项目(SLGYCX1417);陕西省"春笋计划"课题研究项目2014-2015年度

摘　　要：为了研究紫色甘薯醇提物的抗疲劳效应,设计小鼠运动耐力实验,检测小鼠血液生化指标和小鼠骨骼肌核蛋白PGC-1α蛋白表达的影响。结果显示,20mg/m L、10mg/m L组小鼠的运动耐力有显著性延长(P<0.01),表明紫色甘薯醇提物可以提高肌肉的耐力。生化检测结果表明,10mg/m L、20mg/m L组能降低血清尿素、丙二醛、血乳酸的含量(P<0.05),能显著增加肝糖原存储量(P<0.01),显著提高总超氧化物歧化酶的活性(P<0.01)。Western Blot检测结果显示,静止状况下,PGC-1α表达提高了3%;运动状况下,PGC-1α表达提高了6.81%。实验结果表明,紫色甘薯醇提物能提高小鼠体内抗氧化能力,增加糖原含量,降低代谢产物累积,提高PGC-1α蛋白表达来提高小鼠体内能量代谢,达到抗疲劳能力。The study investigated the anti-fatigue mechanism effect of alcohol extracts of purple sweet potato.Movement endurance test is designed for detection of blood biochemical indexes in mice,mice skeletal muscle nucleoprotein PGC- 1α protein expression.The results showed 20 mg/mL,10 mg/mL treated mice have significantly longer time sports endurance（P〈0.01）,which suggested that alcohol extracts of purple sweet potato can increase muscle endurance.Biochemical test results indicate that the 10 mg/mL,20 mg/mL group can reduce the content of blood urea nitrogen（BUN）,malondialdehyde（MDA）,blood lactic acid（LD）（P〈 0.05）,significantly increase liver glycogen storage（P〈 0.01）,significantly improve the activity of total superoxide dismutase（SOD）（P〈 0.01）.Western Blot test showed expression of PGC-1 a increased 3%in static conditions;expression of PGC-1 a increased 6.81%in mice motion situation.Alcohol extracts of purple sweet potato can improve mice antioxidant capacity,increase glycogen,reduce metabolite accumulation,improve PGC- 1α to improve energy metabolism.Therefore,it has the ability of anti- fatigue.

关 键 词：紫色甘薯 过氧化物酶体增殖活化受体γ辅助活化因子1α(PGC-1α) 抗疲劳 

分 类 号：Q955[生物学—动物学]