基于UPLC-Q-TOF/MS技术的北青龙衣不同干燥方法对萘醌类成分影响研究  

Influence of Drying Method on Naphthoquinone Components in Juglans mandshurica Based on UPLC-Q-TOF/MS

作　　者：孙国东 霍金海 洪晓琴 王伟明 SUN Guo-dong HUO Jin-hai HONG Xiao-qin WANG Wei-ming(Institute of Chinese Materia Medica, Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China)

机构地区：[1]黑龙江省中医药科学院中药研究所,黑龙江哈尔滨150036

出　　处：《中药材》2016年第11期2501-2508,共8页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81503348);国家"重大新药创制"科技重大专项(2010ZX09102-138);哈尔滨市青年科技创新人才基金项目(2012RFOYSO42)

摘　　要：目的:研究不同干燥方法对北青龙衣萘醌类成分含量的影响,为建立适合北青龙衣产地加工方法提供参考。方法:采用晒干,阴干,40、50、60℃烘干,冷冻干燥,微波干燥5种方法对北青龙衣样品进行干燥处理。采用UPLC-Triple TOF-MS/MS技术对样品进行测定,数据用Marker View软件进行主成分分析,化合物通过Peak View2.0/masterview1.0软件,依据精确质量数和同位素峰度比确定分子式,再通过对照品及数据库的二级谱图比对、裂解规律分析,结合已有文献报道,确定结构式。结果:不同干燥方法萘醌类有效成分含量发生了明显的变化,鉴定或推断了北青龙衣中24个萘醌类化合物结构,确定了40℃烘干为最适宜干燥方法。结论:该方法快速、准确,可为北青龙衣不同干燥方法的化学成分鉴定与分析提供一种新的策略,对北青龙衣加工生产具有一定的指导意义。Objective：To study the influence of different drying methods on the naphthoquinone components in Juglans mandshurica.Methods：Fresh Juglans mandshurica sample was processed by five drying methods including drying in the sun,drying in the shade,forced air drying with different temperature,freeze drying and microwave drying.Samples of Juglans mandshurica from five drying methods were assessed by UPLC-Triple TOF-MS/MS.The Marker View software was used for data processing and multivariate statistical analysis.The formula of compounds were determined by accurate mass and isotopic abundance ratio from target screening function of Peak View 2.0/masterview1.0 software.Then their structure were determined by analysis of MS/MS fragment or comparison with standard substances and references.Results：Naphthoquinones were the major markers in samples of Juglans mandshurica from different drying methods.24 naphthalenequinones were identified or inferred in Juglans mandshurica.Forced air drying with 40 ℃ was considered as the most suitable drying method.Conclusion：UPLC-Q-TOF/MS method develops a new strategy can identify and analyze chemical constituents from different drying methods rapidly and accurately,which will be serviced in the large scale processing of Juglans mandshurica.

关 键 词：液相色谱-四极杆飞行时间质谱 北青龙衣 干燥方法 萘醌 

分 类 号：R283.1[医药卫生—中药学]