重庆市2011-2016年甲型H1N1流感病毒分离株NA基因变异分析  

Analysis of NA gene variation of influenza A（H1N1） virus isolated in Chongqing from 2011 to 2016

作　　者：陈爽[1] 叶盛[1] 凌华[1] 喻臻[1] 唐云[1] 

机构地区：[1]重庆市疾病预防控制中心微生物检测所,400042

出　　处：《中华实验和临床病毒学杂志》2017年第2期98-103,共6页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 研究重庆市2011-2016年甲型H1N1流感病毒株神经氨酸酶（NA）基因特征,为甲型H1N1流感病毒感染的监测和预防提供试验依据.方法 按分离时间及地点的不同选取43株甲型H1N1流感病毒,经RT-PCR扩增病毒NA基因片段并进行序列测定,进行核苷酸及氨基酸序列分析.结果 基于NA基因的进化树显示,2011-2016年的甲型H1N1流感病毒与疫苗株关系均较近.43株病毒之间的NA基因同源性较高,与疫苗株A/California/07/2009（H1N1）的核苷酸同源性为97.9%~99.4%,氨基酸同源性为96.8%~98.9%.研究中大部分甲型H1N1病毒NA蛋白上都具有疫苗株NA蛋白具有的8个糖基化位点,但也有部分毒株增加42位（NQS）的糖基化位点,或者丢失386位的糖基化位点.结论 甲型H1N1病毒的NA序列变异情况逐年增加,但总体上来说,重庆地区大部分甲型H1N1病毒仍对达菲敏感;同时应加强耐药性监测,为制定应对甲型H1N1流感流行措施提供参考.Objective To research the gene characteristics of neuraminidase（NA） gene of influenza A（H1N1） virus of Chongqing from 2011 to 2016.Methods Forty-three H1N1 influenza virus strains isolated from 2011 to 2016 were chosen according to the different time and places of isolation.NA genetic sections were amplified by RT-PCR and sequenced, and then the sequences of nucleic and amino acid were analyzed.Results Phylogenetic analysis indicated that the relationship between H1N1 viruses from 2011 to 2016 and the vaccine strain was close to each other.Compared with the vaccine strain A/California/07/2009（H1N1）, the homologies in nucleotide and amino acid sequences for NA gene were 97.9%-99.4% and 96.8%-98.9%, respectively.Most of the strains had the same eight potential glycosylation sites as the vaccine strains, and some strains got new potential glycosylation sites at NA protein site 42, while some lost their potential glycosylation sites at NA protein site 386.Conclusions The changes of H1N1 NA gene had increased year by year, but H1N1 viruses in Chongqing area were still sensitive to Daffy.And monitoring the drug resistant viruses should be strengthened, in order to provide measures against H1N1 viruses.

关 键 词：甲型H1N1 流感病毒 序列分析 NA基因 

分 类 号：R440[医药卫生—诊断学] R511.7[医药卫生—临床医学]