RNA恒温扩增法在肺炎支原体检测中的应用  

Application of isothermal RNA amplification in the detection of Mycoplasma pneumoniae

作　　者：周杰[1] 张莉[1] 张鲍虎[1] 黄伟忠[1] 徐康立 张扬[1] 

机构地区：[1]深圳市坪山新区人民医院检验科,广东深圳518118

出　　处：《海南医学》2017年第9期1433-1435,共3页Hainan Medical Journal

基　　金：广东省深圳市坪山新区医疗卫生发展孵化资助项目(编号:201328)

摘　　要：目的探讨RNA恒温扩增法检测肺炎支原体的可行性及其应用价值。方法采集2016年5~7月在我院住院治疗的200例患急性呼吸道感染儿童咽拭子,分别用RNA恒温扩增法和PCR荧光探针法进行肺炎支原体检测,分别计算两种检测方法的检出率,根据公式计算灵敏度和特异性,并进行统计学分析。结果 RNA恒温扩增法检出肺炎支原体17例,检出率为8.5%,PCR荧光探针法检出肺炎支原体18例,检出率为9.0%,两种方法的检出率比较差异无统计学意义(P>0.05);RNA恒温扩增法与PCR荧光探针法相比灵敏度为88.9%、特异度为99.5%。结论 RNA恒温扩增法与PCR荧光探针法检出率相当,具有较高的灵敏度和特异度,可以作为一种新的方法用于肺炎支原体临床检测。Objective To explore the feasibility and clinical value of isothermal RNA amplification in the detection of Mycoplasma pneumoniae. Methods A total of 200 patients with acute respiratory tract infection, who admitted to our hospital from May 2016 to July 2016, were selected as the research subjects. Mycoplasma pneumoniae was detected by the method of isothermal RNA amplification and PCR fluorescence probe respectively. The detection rate of Mycoplasma pneumoniae of the two methods was calculated. The sensitivity and specificity were statistically analyzed according to the specific formula. Results Seventeen cases of Mycoplasma pneumoniae were detected by isothermal RNA amplification method with 8.5% of detection rate; eighteen cases of Mycoplasma pneumoniae were detected by PCR fluorescence probe method with 9.0% of detection rate; there was no significant difference between the two methods in the detection rate（P〉0.05）. Compared to PCR fluorescence probe, the sensitivity and specificity of isothermal RNA amplification were respectively 88.9% and 99.5%. Conclusion Isothermal RNA amplification has nearly equivalent detection rate with PCR fluorescence probe but higher sensitivity and specificity, which can be used as a new method for detecting Mycoplasma pneumoniae clinically.

关 键 词：RNA恒温扩增法 PCR荧光探针法 肺炎支原体 

分 类 号：R563.1[医药卫生—呼吸系统]