大豆GmFDL06基因抗干旱及耐盐性研究  

Study on Drought Resistance and Salt Tolerance of Soybean Gene GmFDL06

作　　者：李媛媛[1] 南海洋[2] 刘宝辉[2] 孔凡江[2] 郭长虹[1] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院/黑龙江省分子细胞遗传与遗传育种重点实验室,黑龙江哈尔滨150025 [2]中国科学院东北地理与农业生态研究所/大豆分子育种实验室,黑龙江哈尔滨150081

出　　处：《大豆科学》2017年第3期351-359,共9页Soybean Science

基　　金：国家自然科学基金(31071445;31171579;31201222和31371643);转基因生物新品种培育重大专项(2016ZX08004-002-003)

摘　　要：bZIP转录因子在植物防卫反应和逆境胁迫应答过程中起着十分重要的作用,大豆GmFDL06是bZIP转录因子家族A组成员,其表达水平受PEG和高盐胁迫影响。本研究分析了GmFDL06转基因大豆苗期的抗干旱和耐盐性。PEG和盐处理后GmFDL06转基因植株的相对植株高度(RPH)和相对植株干重(RSDW)显著高于非转基因大豆东农50。在盐胁迫下,GmFDL06转基因植株比东农50伤害程度低,并且GmFDL06过表达减少了Na^+离子积累,降低了盐胁迫带来的伤害,且过表达GmFDL06促进了下游逆境胁迫基因的表达。这些研究结果表明GmFDL06参与大豆非生物胁迫反应,并有潜力改善大豆的干旱和盐胁迫耐受性,为大豆抗逆基因的研究及抗逆大豆材料的筛选提供了依据。Soybean（ Glycine max L. Merr） is an important oil and high protein crop in the world. However, drought and salt stress can cause soybean yield reduction. Therefore, the study of soybean drought resistance and salt tolerance related gene is very important, bZIP transcription factor plays an important role in the response of plant defense and dversity stress. Soybean GmFDL06 is a member of group A of bZIP transcription factor family, and its transcription level was affected by ABA, PEG and high salt stress, we analyzed the drought resistance and salt tolerance of GmFDL06 transgenic soybean seedling in this study. The relative plant height（RPH） and relative shoot dry weight（RSDW） of GmFDL06 transgenic plants were significant- ly higher than that of wild soybean Dongnong 50 after PEG and salt treatment. Under the salt stress, the damage degree of Gm- FDL06 transgenic plants was lower than Dongnong 50, and over-expression of GmFDL06 reduced the accumulation of Na ＋ ions, reduced the damage caused by salt stress, and the over-expression of GmFDL06 gene could increase the expression of downstream stress related genes such as GmbZIP1, GmNAC11, GmDERB2A ;2, GmWRKY27 and so on. These results indicate that GmFDL06 is involved in the abiotic stress response of soybean and has the potential ability to improve the drought and salt stress tolerance of soybean, which provide the basis for the study of stress related genes and screening stress tolerance soybean cuhivars.

关 键 词：bZIP转录因子 GmFDL06 抗逆性 抗旱性 耐盐性 

分 类 号：S565.1[农业科学—作物学]