大豆炸荚基因GmAGL8的转基因鉴定与功能初探  

Transgenic Identification of Pod-shattering Gene Gm AGL8 in Soybean

作　　者：秦玉涛[1] 王瑞[2] 吕金东[1] 田源[2] 黄天珑 谢皓[1] 郭蓓[2,3] 

机构地区：[1]北京农学院植物科学技术学院,北京102206 [2]北京农学院生物科学与工程学院,北京102206 [3]中华人民共和国农业部华北都市农业重点实验室,北京102206

出　　处：《大豆科学》2017年第3期360-364,共5页Soybean Science

基　　金：国家高技术研究发展计划"863计划"(2012AA101106)

摘　　要：炸荚是大豆的一种自然特征属性,是影响大豆产量的重要因素之一。本研究以前期获得的转大豆炸荚相关基因GmAGL8的T_1代植株为材料,继续繁育获得T_2和T_3代;采用PCR和RT-q PCR检测方法对转基因植株进行基因遗传稳定性和表达情况分析;并以野生型中黄10号为对照,对大豆炸荚性状进行了鉴定分析。结果表明:转基因植株阳性率T_1代为87.5%,T_2和T_3代均达到100%,说明GmAGL8基因已基本能够在转基因后代中稳定遗传。RT-q PCR检测结果显示,转基因植株中GmAGL8基因的相对表达量都明显高于非转基因植株,且各转基因植株之间表达量具有差异性。对T_1、T_2和T_3代植株炸荚率进行了统计,不同世代转基因大豆的平均炸荚率为9.09%,而非转基因大豆炸荚率为83.3%,转基因与非转基因大豆之间炸荚率存在显著差异。综上所述,GmAGL8基因已基本实现在大豆转基因后代中稳定遗传并正常表达,表型鉴定结果初步证明了GmAGL8基因与大豆炸荚性状相关。Pod-shattering is a natural characteristic of soybean, which is one of the critical factors influencing production. In our previous study, we had transformed the gene GmAGL8 （which putative related to soybean pod-shattering） into Glycine max and obtained the T1 generation. In this study, the T2 and T3 generation were multiplied by the T1 generation carrying the GmA- GL8 gene. The genetic stability and expression of transgenic plants were analyzed by using PCR and RT-qPCR methods. As a control group, wild-type Zhonghuang 10 was used to analyze and identify the traits of soybean pods. The results showed the positive rate of the GmAGL8 gene in T1 , T2 and T3 generation were 87. 5%, 100% and 100% respectively. It demonstrated that the GmAGL8 gene can be inherited stably. The results of RT-qPCR analyzing showed the relative expression amount of GmAGL8 gene in transgenic plants was significantly higher than that in non-transgenic plants, and the amounts were various a- mong the transgenie plants. The statistical results for pod-shattering among all plants indicated that the rates of pod-shattering were different among transgenic plants and non-transgenic plants, the average rate of pod-shattering among transgenic plants of all generations was 9. 09%, the rate of pod-shattering among non-transgenic plants was 83.3%. In conclusion, GmAGL8 gene can be inherited stably and expressed normally among transgenic soybean. Furthermore, the phenotypic identification results proved the GmAGL8 gene is related to pod-shattering.

关 键 词：大豆 炸荚 GmAGL8 转基因 

分 类 号：S565.1[农业科学—作物学]