基于高通量测序的大豆连作土壤细菌群落多样性分析  

The Characteristic of Bacterial Community Diversity in Soybean Field with Continuous Cropping Based on the High-Throughput Sequencing

作　　者：朱琳[1] 曾椿淋 李雨青[1] 俞冰倩 高凤[1] 魏巍[2] 许艳丽[3] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]江苏大学农业装备工程学院,江苏镇江212013 [3]中国科学院东北地理与农业生态研究所,黑龙江哈尔滨150081

出　　处：《大豆科学》2017年第3期419-424,共6页Soybean Science

基　　金：国家自然科学基金(41571253);江苏省高校自然科学研究面上项目(15KJB180002);镇江市重点研发计划-现代农业(NY2015023)

摘　　要：研究应用基于Illumina公司的Miseq高通量测序平台,深度解析东北黑土大豆短期连作和长期连作土壤细菌群落结构多样性。通过对细菌16S rRNA序列V4区的高通量测序,短期(3年)和长期连作(20年)大豆田土壤分别得到180 980和221 424条有效序列,注释为1 254和1 432个细菌可操作分类单元(operational taxonomic unit,OTU),且长期连作土壤细菌群落丰富度和多样性均高于短期连作土壤。在细菌门分类水平上,短期和长期连作土壤中细菌优势菌群构成为相同的8个细菌菌门(所占比例>1%),依次包括变形菌门、酸杆菌门、疣微菌门、厚壁菌门、拟杆菌门、放线菌门、芽单胞菌门和硝化螺菌门,其所占总比例之和分别达到细菌菌门总数的85.5%和86.3%。在细菌属分类水平上,短期和长期连作土壤中细菌TOP10优势菌群构成相同,包括疣微菌门的Spartobacteria属、酸杆菌门的Gp1、Gp4、Gp3和Gp6属、芽单胞菌门的Gemmatimonas属、硝化螺菌门的Nitrospira属、变形菌门的Sphingomonas属和Bradyrhizobium属以及厚壁菌门的Bacillus属,且10个细菌菌属所占比例之和分别达到细菌菌门总数的71.3%和69.0%。结果表明:东北黑土区大豆经过长期连作后土壤细菌优势菌菌群结构变化较小,但群落丰富度和多样性较短期连作略有增加,且对大豆养分吸收和生长有促进作用的根瘤菌Bradyrhizobium属和硝化细菌Nitrospira属所占比例增加。研究结果对解释大豆长期连作根病抑制性土壤形成机制具有一定价值。Based on the Miseq platform of Illumina company, we explored the effect of short-term and long-term copping con- tinuous with soybean on the composition diversity of the bacterial community. According to the sequencing of the V4 region of bacterial 16S rRNA gene, we obtained 180 980 and 221 424 efficient sequences, respectively, and confirmed them as 1 254 and 1 432 OTUs. The richness and diversity of bacterial community form the soybean field with long-term continuous cropping was higher than those in soybean field with short-term continuous cropping. At the phylum level, there were 8 bacterial phy- lum as the dominant group in both fields （the ratio to total above 1% ）, including Proteobacteria, Acidobacteria, Verrucomi- crobia, Firmicutes, Bacteroidetes, Actinobacteria, Gemmatimonadetes and Nitrospira. All of which were 85.5% and 86. 3% of the total community in short and long-term continuous cropping fields, respectively. At the genus level, the top 10 groups were the same bacterial genera between the both treatments, including Spartobacteria in Verrucomicrobia, Gpl, Gp4, Gp3 and Gp6 in Acidobacteria, Gemmatimonas in Gemmatimonadetes, Nitrospira in Nitrospira, Sphingomonas and Bradyrhizobium in Proteobacteria, and Bacillus in Firmicutes. All of these genera were 71.3% and 69. 0% of the total community in each field, respectively. The results of this study indicated that the richness and diversity of bacterial community in soybean field was in- creased after long-term continuous cropping, although there was just a little change in bacterial community composition, espe- cially those of dominant groups. It is of great significance to explain the mechanism of the formation of suppressive soil for soy- bean root disease by long-term continuous cropping.

关 键 词：大豆 长期连作 Miseq 细菌群落 结构多样性 

分 类 号：S154.3[农业科学—土壤学] S565.1[农业科学—农业基础科学]