大豆种质资源对尖镰孢菌根腐病的抗性鉴定及生理分析  

Identification for Resistance to Root Rot Caused by Fusarium Oxysporum in Soybean Germplasm and Physiological Analysis

作　　者：张婵娟[1] 廖胜泉 宋欢[1] 赵雪[1] 韩英鹏[1] 刘琦[2] 李文滨[1] 武小霞[1] 

机构地区：[1]东北农业大学大豆生物学教育部重点实验室/农业部北方大豆生物学与遗传育种区域重点实验室,黑龙江哈尔滨150030 [2]黑龙江省农业科学院生物技术研究所/作物与家畜分子育种重点实验室,黑龙江哈尔滨150086

出　　处：《大豆科学》2017年第3期441-446,共6页Soybean Science

基　　金：国家自然科学基金(31301339);黑龙江省教育厅科学技术研究项目(12541049);黑龙江省教育厅新世纪项目优秀人才项目资助(1253-NCET-005);教育部博士点项目(20122325120012);黑龙江省自然科学基金面上项目(C201130)

摘　　要：对来自全国19个省份及国外的342份大豆种质资源进行鉴定,其中主要包含来自黑龙江的95份、吉林的21份、辽宁的19份、内蒙的6份、北京的23份、河北的12份、安徽的3份、美国的24份及其它各省份的大豆种质,以尖镰孢菌强毒菌株M38为供试菌株,采用菌层接种法,筛选得到不同表现的抗源材料,并选取一对具有代表性的抗感品种用菌液处理后测定其幼根内超氧化物歧化酶(SOD)、可溶性糖的含量、丙二醛(MDA),从而为分子辅助育种奠定基础。鉴定结果表明:鉴定筛选出高抗(HR)大豆种质10个,但没有发现免疫品种;对大豆资源的来源分析表明:北方的抗病种质资源较南方丰富;对生化指标测定结果表明,用菌液处理后抗感品种的SOD的活性先上升后下降,可溶性糖的含量整体呈下降趋势,但抗病品种的活性及含量均高于感病品种,抗病品种MDA变化幅度明显小于感病品种。In the present study, a total of 342 soybean germplasm from 19 provinces and foreign countries were evaluated for the resistance to F. Oxysporum with the method of changed bacteria layer inoculation. The 19 provinces mainly contain 95 re- sources from Heilongjiang, 21 resources from Jilin, 19 resources from Liaoning, 6 resources from Inner Mongolia, 23 re- sources from Beijing,12 resources from Hebei, 3 resources from Anhui and 24 resources from American. SOD, soluble sugar, and MDA were studied after seeding root of resistant and susceptible soybean were treated by microbial from F. Oxysporum. In order to lay the foundation for molecular assisted breeding. The appraisal result showed that ten soybean accessions with high resistance to F. Oxysporum were identified and none accession were found to be immune to F. Oxysporum. The result of source showed that resistance resources from the north are richer than the south. The result of biochemical showed that after inocula- ted by microbial in two varieties. The activity of SOD raised first and downward trend. The content of soluble sugar decreased and they were much higher in resistant variety than in susceptible one, the change of MDA in resistant obviously smaller than the susceptible one.

关 键 词：大豆根腐病 尖镰孢菌 抗病鉴定 病情指数 生化指标 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]