大豆菌核病鉴定方法研究进展  

Research Development of Identification Methods in Soybean White Mold

作　　者：孙明明[1,2] 韩英鹏[1] 赵雪[1] 王萍[2] 吕世翔[2] 李智媛[2] 王冠[2] 李文滨[1] 

机构地区：[1]东北农业大学大豆生物学教育部重点实验室/东北农业大学农业部东北大豆生物学与遗传育种重点实验室,黑龙江哈尔滨150030 [2]黑龙江省农业科学院信息中心,黑龙江哈尔滨150086

出　　处：《大豆科学》2017年第3期470-474,共5页Soybean Science

基　　金：黑龙江省自然科学基金面上项目(C2015011)

摘　　要：大豆菌核病在全球范围内均有发生,由于病原菌寄主范围较广,难于防治,对大豆产量和品质产生严重影响。对于大豆菌核病抗性种质资源的鉴定是抗性机理研究、基因定位及抗病育种工作的重要基础,文章对大豆菌核病资源鉴定中涉及的接种体(菌丝、孢子和菌核)的培养、植株不同部位的接种鉴定、草酸鉴定、茎中可溶性色素水平测定及田间鉴定方法进行了系统的总结和分析,并针对接种体的选择、环境条件对发病的影响及田间与温室鉴定结果不一致等问题展开讨论,为大豆菌核病抗性资源的筛选,菌核病发病机理及抗性基因的定位研究奠定基础。Soybean white mold （ Sclerotinia sclerotiorum, SWM） caused by Sclerotinia sclerotiorum （ Lib. ） de Bary is a world- wide soybean disease. The pathogen has a extensive host range, so it is difficult to prevent and causes serious yield and quality loss every year. The SWM resistance identification of soybean germplasm is the foundation of resistance mechanism research, gene location and resistant breeding. We summarized and analyzed the inoculum（ mycelium, ascospore, and sclerotia）, and different identification methods （different plant parts inoculation, oxalic acid identification, soluble pigments content, and field identification）, and discussed the choices of inoculum, environment factors influence and different results between growthroom and field identification. This paper could supply reference for SWM resistant germplasm screening, disease mechanism and re- sistant gene location.

关 键 词：大豆菌核病 核盘菌 鉴定 接种体 环境条件 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]