金叶锦带组培技术  

Tissue Culture Technique for Weigela 'Red Prince'+vat

作　　者：马金贵[1] 郭淑英[1] 

机构地区：[1]唐山职业技术学院农林工程系,河北唐山063004

出　　处：《北方园艺》2017年第10期92-94,共3页Northern Horticulture

基　　金：河北省科技厅科学研究计划资助项目(15236830)

摘　　要：以金叶锦带的腋芽为外植体,采用组织培养法,通过在培养基中添加不同浓度激素,筛选最佳培养基,为金叶锦带的大面积推广提供技术支撑。结果表明:最佳诱导培养基为MS+0.6mg·L^(-1) 6-BA+0.1mg·L^(-1) NAA+蔗糖30g·L^(-1)+琼脂7g·L^(-1),诱导率达86.3%;最佳增殖培养基为MS+1.0 mg·L^(-1) 6-BA+1.0 mg·L^(-1) GA3+蔗糖30g·L^(-1)+琼脂7g·L^(-1),增殖系数7.250 6;最佳生根培养基为1/2MS+1.0mg·L^(-1) IBA+蔗糖30g·L^(-1)+琼脂7g·L^(-1),生根率100.0%。Buds of Weigela ‘Red Prince’＋vat were used as explants.By using tissue culture methods，the optimum medium for Weigela ‘Red Prince’＋vat were selected via adding different concentrations of hormone,to provide technique reference for promotion of Weigela‘Red Prince’＋vat.The results showed that the inducing culture medium of MS＋0.6 mg·L^-1 6-BA＋0.1 mg·L^-1 NAA＋sucrose 30 g·L^-1＋agar 7 g·L^-1 was optimal for inducement，the inducement rate was 86.3%;the best proliferation medium was MS＋1.0 mg·L^-1 6-BA＋1.0 mg·L^-1 GA3＋sucrose 30 g·L^-1＋agar 7 g·L^-1，the proliferation times was 7.250 6;the best rooting medium formula was 1/2MS＋1.0 mg·L^-1 IBA＋sucrose 30 g·L^-1＋agar 7 g·L^-1,the rooting rate was 100.0%.

关 键 词：金叶锦带 组织培养 激素配比 

分 类 号：S687.903.6[农业科学—观赏园艺]