“鲁加5号”柱型苹果胚状体再生体系优化  

Optimization of Embryoid Regeneration System From 'Lujia No.5' Columnar Apple

作　　者：周爱琴[1] 祝军[1] 杨庆山[2] 王振猛[2] 李永涛[2] 魏海霞[2] 

机构地区：[1]青岛农业大学,山东青岛266109 [2]山东省林业科学研究院,山东济南250014

出　　处：《北方园艺》2017年第10期95-99,共5页Northern Horticulture

基　　金：国家现代苹果产业技术体系资助项目(CARS-28-01-07);国家"十二五"科技支撑计划资助项目(2013BAD02B01);山东省良种产业化工程资助项目;青岛市民生科技计划资助项目(14-2-3-38-nsh);青岛市现代果树产业技术体系资助项目(6622316107)

摘　　要：利用胚状体再生植株,可以获得遗传稳定性好且数量多的再生植株,这对于建立高频稳定的遗传转化体系具有重要意义。该试验以苹果新品种"鲁加5号"为试材,采用叶片再生方法,研究了其胚状体再生体系,为建立遗传转化体系奠定了基础。结果表明:MS+6-BA1.0mg·L^(-1)+NAA 0.20mg·L^(-1)为"鲁加5号"最适宜的继代增殖培养基。叶片的最佳放置方式为近轴面接触培养基。胚胎发育阶段最佳暗培养时间为10d。最佳体细胞胚诱导培养基为MS+6-BA 1.0mg·L^(-1)+NAA 5.0mg·L^(-1)+TDZ 0.5mg·L^(-1)。胚胎发育阶段,通过胚状体不同时期形态学组织学观察,显示胚状体发育时期经历原胚、球形胚、心形胚、鱼雷形胚、子叶胚和成熟胚阶段。It is very important to establish a stable genetic transformation system with high frequency stability，which can be used to regenerate plants with the somatic embryogenesis.In this experiment，new variety of apple‘Lujia No.5’was used as test material，the regeneration system of embryoid bodies was studied，which laid the 〖JP〗foundation for the establishment of genetic transformation system.The results showed that MS＋6-BA 1.0 mg·L^-1＋NAA 0.20 mg·L^-1 was the most suitable subculture medium.It was considered that the best placed mode was adxial surface touching medium.The best time length of dark culture was 10 days.The best embryo cell media was MS＋6-BA 1.0 mg·L^-1＋NAA 5.0 mg·L^-1＋TDZ 0.5 mg·L^-1.During embryonic development，the embryos in different periods of morphological and histological observation，results showed that the embryoids experienced proembryonal,globular embryo，heart-shaped embryo，torpedo shaped embryo，cotyled onary embryo and mature embryos stages.

关 键 词：胚状体 再生 “鲁加5号” 优化 

分 类 号：S661.103.6[农业科学—果树学]