大兴安岭火烧迹地土壤真菌群落结构及多样性研究  

Study on Diversity and Community Characteristics of Soil Fungi at Burned Forest Sites for the Great Xing′an

作　　者：张虹[1] 峥嵘[1] 

机构地区：[1]内蒙古师范大学生命科学与技术学院,内蒙古呼和浩特010022

出　　处：《北方园艺》2017年第10期159-166,共8页Northern Horticulture

基　　金：内蒙古自治区科技计划资助项目(20130435)

摘　　要：以大兴安岭火烧迹地在不同火烧年和不同火烧程度的土样为研究对象,揭示大兴安岭火烧迹地恢复过程中土壤真菌的多样性与群落变化特征,为火烧后森林生态系统的科学管理和森林更新提供理论依据。用PCR-DGGE法对土壤真菌进行了分析,采用Quantity one软件分析其多样性,通过序列的同源性比较构建系统发育树,真菌结构主成分分析采用Canoco等软件。结果表明:每个样品真菌种类丰富,不同样品有相同的真菌菌属也有不同的真菌菌属,17个优势条带测序所得菌属与黏滑菇属(Hebeloma)、蜡伞属(Hygrophorus)、蜡壳菌属(Sebacima)、管柄囊霉属(Funneliformis)亲缘关系最近,其中还有一些不可培养的真菌。不同火烧年和火烧程度之间土样真菌香农指数、丰富度、均匀度均达到显著差异(P<0.05)。不同火烧程度、不同火烧年均能影响土壤真菌的群落结构。There were soil samples of different burned year and different burned degree to study at burned forest site for Great Xing′an,which would reveal diversity of soil fungi and variational characteristic of community,and provide theoretical basic for the science management of forest ecosystem and forest regeneration.The soil fungal characteristics were analyzed by PCR-DGGE method.Quantity one was used to analyze the diversity，and compared sequence homology to construct phylogenetic tree.Principal component analysis of fungi were analyzed using Canoco software.The results showed that each fungus was rich in species，of which had the same fungus species and different fungus species.and the 17 preponderant bands had a close relationship with Hebeloma，Hugrophorus，Sebacima，Funneliformis，and some uncultured fungi.There was a significant influence on different soil fungal Shannon index（H），richness（S），evenness（EH） in the different year and different burned degree（P〈0.05）.And different burned degree and different burned year also influenced soil fungal structure.

关 键 词：大兴安岭 火烧迹地 土壤真菌 群落结构 多样性 

分 类 号：S718.8[农业科学—林学]