磁介导功能型纳米粒子在铁支架表面定向捕获CD34阳性细胞  

Magnetism induced functional nanoparticles for capturing CD34+ cells in situ on the surface of iron stent

作　　者：范仪 李萍[1] 赵雅娟[1] 涂秋芬[1] 黄楠[1] 

机构地区：[1]西南交通大学材料先进技术教育部重点实验室,成都610031 [2]西南交通大学生命科学与工程学院,成都610031

出　　处：《中国科学：技术科学》2017年第5期540-546,共7页Scientia Sinica(Technologica)

基　　金：国家自然科学基金(批准号:81271701)资助项目

摘　　要：构建内皮祖细胞捕获支架是目前心血管疾病治疗的一个研究热点.利用磁性纳米粒子捕获内皮祖细胞也越来越受到关注,但是单纯的磁性纳米粒子和磁场源地作用相对微弱,本研究引入铁支架作为另一磁响应源,共同作用来提高纳米粒子表面捕获率.首先利用CD34抗体构建了一种定向捕获CD34阳性细胞的功能型磁性纳米粒子,再利用外加磁场将其归巢到铁支架表面.FTIR和TEM结果表明CD34抗体已成功接枝到双羧基PEG包裹的Fe_3O_4纳米粒子上,体外细胞评价证实该纳米粒子不具有细胞毒性.体内动物实验结果表明该Fe_3O_4-PEG@CD34纳米粒子能够特异性识别捕获CD34阳性细胞,并且在外加磁场的作用下能够将其快速归巢到铁支架表面,这为实现支架表面快速内皮化提供了可能.Constructing endothelial progenitor cell （EPC） capturing stents is currently hot field in cardiovascular diseases treatment. The use of magnetic nanoparticles capturing endothelial progenitor cells becomes more and more popular, but magnetic nanoparticles and magnetic field source function are relatively weak. This study introduced the iron stent as another source of the magnetic response to work together to improve the nanoparticles surface capture rates. Firstly, CD34 antibody-modified magnetic nanoparticles （Fe304-PEG@CD34） were synthetized with the aim of specifically capturing CD34＋ cells, and then were homed to the surface of iron stems with the additional magnetic field. FTIR and TEM results showed that CD34 antibody has been successfully grafted onto Fe3O4-PEG magnetic nanoparticles. In vitro cell evaluation confirmed that the nanoparticles don＇t have cytotoxicity. The results of in vivo animal experiment exhibited that the Fe304-PEG@CD34 nanoparticles could specificity recognize and capture CD34＋ cells. The Fe3O4-PEG@CD34 nanoparticles could rapidly home CD34＋ cells to the surface of iron stents with the additional magnetic field. Thus, the CD34 antibody-modified magnetic nanoparticles might help to realize rapid endothelialization.

关 键 词：内皮祖捕获 干细胞 磁性纳米粒子 CD34抗体 

分 类 号：R54[医药卫生—心血管疾病] TB383.1[医药卫生—内科学]