何首乌饮对衰老大鼠生精细胞线粒体凋亡通路关键基因表达的影响  

Effect of Heshouwuyin on regulating the key genes expression of mitochondrial apoptotic pathway in aging rats' spermatogenic cells

作　　者：安福丽[1] 王玉娟[1] 刘昊坤[2] 杜云帆[2] 秦瑄希 牛嗣云[3] 高福禄[4] AN Fu-li WANG Yu-juan LIU Hao-kun DU Yun-fan QIN Xuan-xiz NIU Si-yun GAO Fu-lu(Department of Pharmacy,Chinese Traditional Medicine Hospital of Hebei Province, Shijiazhuang 050011, China Military Transportation University of PLA , Tianjin 300000, China Department of Embryology Organization, Hebei University, Hebei Baoding 071000, China School of Life Science, Hebei Normal University, Shijiazhuang 050024, China)

机构地区：[1]河北省中医院药学部,石家庄050011 [2]中国人民解放军军事交通学院九连,天津300000 [3]河北大学医学院组织学胚胎学教研室,河北保定071000 [4]河北师范大学生命科学学院,石家庄050024

出　　处：《解剖学报》2017年第3期287-295,共9页Acta Anatomica Sinica

基　　金：国家自然科学基金(81673714)

摘　　要：目的探讨何首乌饮对衰老大鼠睾丸生精细胞线粒体凋亡通路关键基因表达的影响。方法选用12月龄Wistar雄鼠45只,随机分为3组:青年对照组、自然衰老组、何首乌饮组,每组15只,何首乌饮组和自然衰老组于16个月开始分别灌胃何首乌饮和等量蒸馏水,连续60d。青年对照组于12月龄,其余两组于18月龄分别进行实验,通过Real-time PCR、免疫荧光染色和免疫印迹法,观察各组大鼠睾丸组织线粒体凋亡通路关键基因DR6、BAX、Caspase-3、Cyt-C、14-3-3σ的表达变化。结果自然衰老大鼠线粒体凋亡通路关键基因14-3-3σ表达明显低于青年对照组,DR6、BAX、Caspase-3、Cyt-C表达明显高于青年对照组;而何首乌饮用药后能明显逆转上述现象。结论何首乌饮提高衰老大鼠精子质量与线粒体凋亡通路有关。Objective To investigate the effect of Heshouwuyin on regulating the key genes expression of mitochondrial apoptotic pathway in aging rats＇ spermatogenic cells. Methods Forty-five 12-month-old male Wistar rats were randomly divided into three groups： young control group（YCG） , natural aging group（NAG） and Heshouwuyin group （SWYG）, 15 rats per group. From 16-month-old, SWYG and NAG were intragastrically given Heshouwuyin and equal amount of normal saline respectively. Gavage treatment kept for 60 days. YCG at 12-month-old and the other two groups at 18-month-old entered experiment respectively. Real-time PCR, immunofluorescenee staining, and Western blotting were used to detect the expression of DR6, BAX, Caspase-3, Cyt-C and 14-3-3ty. Results The expression of 14-3-3ty, a key factor in the mitoehondrial apoptotie pathway, was significantly lower and the expressions of DR6, BAX, Caspase-3, and Cyte were significantly higher in the NAG than in the YCG, but intervention with Heshouwuyin significantly reversed this phenomenon. Conclusion Heshouwuyin improves the spermatogenic function of the testes by regulating expression of key genes in the mitoehondrial apoptosis pathway.

关 键 词：何首乌饮 睾丸 线粒体凋亡通路 实时定量聚合酶链反应 免疫荧光 免疫印迹法 衰老 大鼠 

分 类 号：Q189[生物学—神经生物学]