姜黄素对肝癌HepG2细胞增殖及凋亡的影响  

The effect of curcumin on hepatocellular carcinoma HepG2 cells' proliferation and apoptosis

作　　者：曹聪[1] 李广志[2] 胡高裕 覃小珊[1] 周喜汉[1] 黄赞松[1] 

机构地区：[1]广西右江民族医学院附属医院消化内科,百色533000 [2]广西右江民族医学院附属医院重症医学科,百色533000

出　　处：《广西医科大学学报》2017年第6期813-815,共3页Journal of Guangxi Medical University

基　　金：广西自然科学基金资助项目(No.2014GXNSFAA83038)

摘　　要：目的:探讨中药姜黄素对肝癌HepG2细胞增殖及凋亡的影响。方法:将肝癌HepG2细胞分为姜黄素组与空白对照组,采用四甲基噻唑氮蓝(MTT)比色法检测不同浓度(10μmol/L、20μmol/L、40μmol/L、60μmol/L和80μmol/L)姜黄素处理后HepG2细胞的增殖抑制率,流式细胞术检测姜黄素对HepG2细胞周期及细胞调亡的影响。结果:不同浓度姜黄素组中,随着药物浓度增加,作用时间延长,细胞的增殖抑制率升高,组间比较差异具有统计学意义(P<0.05)。与空白对照组比较,80μmol/L姜黄素组S期细胞比例显著增加,细胞凋亡率明显升高,差异均有统计学意义(均P<0.05)。结论:姜黄素可以有效抑制肝癌HepG2细胞的增殖,并促进细胞凋亡,其抑癌作用可能与阻滞细胞于S期有关。Objective：To explore the effect of traditional Chinese medicine curcumin on HepG2 live cancer cells proliferation. Methods： The HepG2 cells were divided into acurcumin group and a control group. The inhibitory rate of proliferation of HepG2 cells treated with different concentration of curcumin （10, 20, 40, 60 and 80 μmol/L） were analyzed by MTT assay. The influence of curcumin on HepG2 live cancer cells cycle and cells apoptosis were analyzed by flow cytometry. Results： In the different concentrations of curcurain group, the inhibitory rate was increased with the increase of drug concentration and the exposure time, and the difference among groups were statistically significant （ P 〈 0. 05）. Compared with the control group, the proportion of S phase cells in 80 μmol/L curcumin group was significantly increased, the apoptosis rate was significantly increased （ P〈0.05）. Conclusion： Curcumin could inhibit the proliferation of hepatocellular carcinoma HepG2 cells and accelerate the apoptosis via the inhibition of cell cycle.

关 键 词：姜黄素 肝癌HEPG2细胞 细胞调亡 

分 类 号：R375.7[医药卫生—病原生物学]