正交设计法优选胆石六号汤提取工艺  

Optimization of Extraction Process for Gallstone VI Decoction by Orthogonal Test

作　　者：刘燕[1] 陈积[1] 胡姗姗[2] 陈泽慧[2] 田应彪[2] 

机构地区：[1]遵义医学院药学院,贵州遵义563003 [2]遵义医学院附属医院,贵州遵义563003

出　　处：《中国现代中药》2017年第5期711-715,共5页Modern Chinese Medicine

基　　金：贵州省教育厅自然科学研究项目[黔教合K字(2012)054号];贵州省科技计划课题[黔科合SY字(2015)3028号];贵州省药剂学研究生卓越人才培养计划[黔教研合ZYRC字(2014)019]

摘　　要：目的:优选胆石六号汤的最佳提取工艺。方法:以总黄酮、栀子苷含量及干膏率的综合评分为指标,在单因素试验基础上,通过L_9(3~4)正交试验考察加水量、提取时间和提取次数对胆石六号汤水提工艺的影响。结果:最佳提取工艺为加12倍量水,煎煮3次,每次1 h。结论:优选的提取工艺合理可行,为胆石六号颗粒剂的制备提供参考。Objective： To optimize the extraction process of gallstone VI decoction. Methods： The comprehensive scores of total flavonoids, geniposide content and dry extract rate were used as indicators, the effect of amount of water, ex- traction time and frequency on gallstone VI of water extraction process were observed on the basis of single factor tests through L9 （34） orthogonal design. Results： The optimum process was determined as follows： adding 12 times of water and extracting 1 h for 3 times. Conclusion： This optimized process is reasonable and feasible, which could guide industrial production.

关 键 词：胆石六号汤 总黄酮 栀子苷 干膏率 正交试验 

分 类 号：R282.71[医药卫生—中药学]