不饱和脂肪酸对人胃癌细胞蛋白激酶B信号通路的调控作用  

Regulating action of unsaturated fatty acids on human gastric cancer cells protein kinase B signaling pathways

作　　者：俞发荣[1] 陈望军[1] 雷中坚[1] 连秀珍[1] 谢明仁[1] 李登楼[1] 杨博[1] 郭蕴萱 

机构地区：[1]甘肃政法学院甘肃省证据科学技术研究与应用重点实验室,兰州730070

出　　处：《中国临床药理学杂志》2017年第10期897-900,共4页The Chinese Journal of Clinical Pharmacology

基　　金：甘肃省基础研究创新群体基金资助项目(145RJIA333);兰州市科技计划基金资助项目(2014-1-182;2015-3-80);甘肃省高校科技创新团队基金资助项目(2016C-09);兰州市人才创新创业基金资助项目(2016-RC-2);西北民族地区侦查理论与实务研究中心基金资助项目;甘肃政法学院重大项目基金资助项目(2016XZD15)

摘　　要：目的研究不饱和脂肪酸对人胃癌细胞蛋白激酶B(PKB/Akt)信号通路的调控作用。方法取胃癌患者肿瘤组织,移植到重症联合免疫缺陷(SCID)小鼠上,将移植瘤组织酶解消化,分离出细胞进行传代培养。然后将传代细胞分为5组:低中高3个浓度实验组(不饱和脂肪酸0.3,0.6,1.2 mg·L^(-1)),对照组(5-氟尿嘧啶10 mg·L^(-1)),溶剂对照组(二甲基亚砜30μL·L^(-1)),给药后培养48 h。用噻唑蓝(MTT)法检测不饱和脂肪酸对人胃癌细胞的增殖抑制作用,用酶联免疫吸附法检测胃癌细胞中p21、双微体基因(MDM2)及PKB/Akt表达水平。结果高中低3个浓度实验组人胃癌细胞增殖抑制率比对照组分别升高了31.7%,55.51%,68.28%;这3组的p21和MDM2水平比对照组分别升高了4.39%,80.12%,161.36%和19.81%,97.76%,207.35%;这3组的PKB/Akt水平比对照组降低了13.31%,57.69%,78.71%。结论不饱和脂肪酸对人胃癌细胞增殖的抑制作用主要与升高肿瘤细胞内p21与MDM2水平和抑制PKB/Akt信号通路的活性有关。Objective To study the regulating action of unsaturated fatty acids on human gastric cancer cells protein kinase B （PKB/Akt）signaling pathways. Methods The gastric cancer tissue of adult patients with gastric cancer was isolated and transplantation in severe combined immunodeficiency （SCID） mice. Separation cells from gastric cancer trans- planted tumor by digestive enzyme were cultured. They were divided into five groups ： three doses experimental groups （ unsaturated fatty acid 0. 3, 0. 6, 1.2 mg·L^-1 ）, control group （5 - fluorouracil 10 mg·L^-1 ） , sol- vent control group （ dimethyl sulfoxide 30 μL·L^-1 ）, cultivate 48 h alter the treatment. The toxic effects of the unsaturated fatty acid on human gastric cancer cells were detected by 3 - （4,5 -dimethyl -2 -thiazolyl） -2,5 - diphenyl - 2 - H - tetrazolium bromide （ MTT ） method. The levels of p21 , murine double minute2 （ MDM2 ） , PKB/Akt in tumor ceils were detected by the method of enzyme linked immunosorbent assay. Results Compared to the control group, the human gastric cancer cells proliferation inhibition rate in high, moderate, low, doses experi- mental groups were increased by 31.7%, 55.51%, 68.28%, respectively. The levels of p21 and MDM2 in that three groups were increased by 4. 39%, 80. 12%, 161.36% and 19.81%, 97.76% ,207.35%, respectively. The level of PKB/Akt in that three groups was reduced by 13.31%, 57.69%, 78.71%, respectively. Conclusion The unsaturated fatty acids on human gastric cancer cells proliferation inhibition mainly related to elevated p21, MDM2 levels within the tumor ceils, inhibit the activity of PKB/Akt signal pathway.

关 键 词：不饱和脂肪酸 人胃癌细胞 重症联合免疫缺陷小鼠 

分 类 号：R979.1[医药卫生—药品]