丙泊酚静脉麻醉对大鼠水杨酸钠排泄的影响  

Effect of propofol intravenous anesthesia on the excretion of sodium salicylate in rats

作　　者：高洪波[1] 徐继辉[1] 贾金雪 缪文禹 秦金东[1,2] 李学峰[1,2] 陈彪[3] 薛永志[1,2] 

机构地区：[1]包头医学院药理学教研室,内蒙古包头014060 [2]包头医学院药物代谢与肝脏分子药理研究所,内蒙古包头014060 [3]包头医学院第一附属医院麻醉科,内蒙古包头014060

出　　处：《中国临床药理学杂志》2017年第10期913-916,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(30760289;81460567);内蒙古自然科学基金资助项目(2009MS1104;2014MS0813);内蒙古教育厅基金资助项目(NJ03148;NJZY13244);内蒙古卫生厅基金资助项目(201302099)

摘　　要：目的研究丙泊酚全身麻醉过程对水杨酸钠排泄的影响及其机制。方法麻醉前,取雄性SD大鼠16只,腹腔注射1%水杨酸钠80 mg·kg^(-1)后,收集尿液,记录实际排尿时间和尿量。用光谱法测定尿液中水杨酸浓度,绘制尿药浓度-时间曲线。经3 d清洗期,上述大鼠尾静脉注射丙泊酚150 mg·kg^(-1)后,立即腹腔注射水杨酸钠,方法同前。绘制麻醉前与麻醉后尿药浓度-时间曲线,代入药代动力学分析软件3P97,计算尿药代动力学参数,比较麻醉前与麻醉后水杨酸钠排泄能力的变化。眶静脉丛取血检测血气指标,并进行血气指标与水杨酸累积排泄量相关分析。结果丙泊酚麻醉前与麻醉后,p H分别为7.44±0.04,7.37±0.06;二氧化碳分压(PCO2)分别为(39.58±6.42),(47.71±6.80)mm Hg;氧分压(PO2)分别为(88.25±6.80),(67.75±3.73)mm Hg;剩余碱(BE)分别为(1.20±1.05),(-5.08±1.88)mmol·L^(-1);最大排泄率(Rmax)分别为(0.62±0.15),(0.39±0.04)mg·h^(-1);Cmax分别为(1.43±0.23),(0.85±0.15)mg·kg^(-1);0~28 h排泄-时间曲线下面积(ERUC0-28)分别为(25.83±3.8),(17.35±2.01)mg·h·L^(-1),差异有统计学意义(均P<0.05)。p H、PO2、BE与水杨酸累积排泄量呈明显正相关,相关系数(R2)分别为0.9758,0.9760,0.9398;而PCO2与水杨酸累积排泄量呈明显反正相关,R2为0.9023,差异有统计学意义(P<0.05)。结论丙泊酚静脉麻醉能减慢大鼠水杨酸钠排泄速度,与血气指标显著相关。Objective To explore the effects of propofol intravenous anesthesia on the excretion of sodium salicylate and its mechanism in rats. Methods A total of 16 male SD rats were placed in rat metabolic cage to Collect urine after intraperitoneal injection of 1% sodium salicylate 80 mg·kg^-1. Then the actual time urination and urinary volume were recorded. And salicylic acid concentration in urine were determine by spectrometry. Urine drug concentration - time curve were drawed. After 3 days of cleaning period, the rats were given intravenous injection of propofol 150 mg·kg^-1 immediately after intraperitoneal injection of sodium salicylate with the former method. Urine drug concentration-time curve were drewed before and after anesthesia. Urine drug metabolism and pharmacokinetics parameters were analyzed with 3P97 Software. Sodium salicylate excretion capacity was analyzed before and after anesthesia. In experiment 3 h, the blood gas indexes were detected in the orbital venous plexus. After 3 h, blood from orbital venous was collected for analyzing the parameters of blood gas. And then, blood gas index and salicylic acid accumulation correlation analysis was eliminated. Results pH were 7.44 ± 0. 04,7. 37 ± 0. 06 respectively before or after anesthesia by propofol intravenous. Partial pressure of carbon dioxide （ PCO2 ） were （ 39. 58 ± 6. 42 ）, （47. 71± 6. 80 ） mmHg respectively. Partial pressure of oxygen （ PO2 ） were （ 88. 25 ± 6. 80 ）, （ 67. 75 ± 3.73 ） mmHg respectively. Base excess （BE） were （1.20 ± 1.05）, （ - 5.08 ± 1.88） mmol·L^-1 respectively. Maximum excretory rate （Rmax） were （0. 62 ± 0. 15 ）, （0. 39 ± 0. 04） mg·h^-1 respectively. Cmax were （ 1.43 ± 0. 23 ）, （ 0. 85 ± 0. 15 ）mg·kg^-1 respectively. Excretion under curve （ ERUC 0-2s ） were （25.83 ± 3.8 ）, （ 17.35 ± 2. 01 ） mg·h·L^-1 respectively, the difference was statistically significant （ all P 〈 0. 05）. Statistically significant positive correlation was presente

关 键 词：丙泊酚 血气分析 水杨酸钠 药代动力学 

分 类 号：R971.2[医药卫生—药品]