柴芪汤对代谢综合征大鼠血管损伤预防及治疗的干预机制研究  

Protective and Therapeutic Mechanism of Chaiqi Decoction on Vascular Injury of MS Rats

作　　者：陈丽如[1] 刘源[1] 张嘉琰 彭龙[1] 张立平[2] 

机构地区：[1]北京中医药大学东方医院,北京100078 [2]北京中医药大学国际交流与合作处,北京100029

出　　处：《中医药信息》2017年第3期39-44,共6页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81273695);高等学校博士学科点专项科研基金项目(No.20130013110016)

摘　　要：目的:观察柴芪汤对饮食诱发的代谢综合征(MS)模型大鼠血管损伤的预防及治疗的干预效果,探讨其血管保护机制。方法:将56只SPF级雄性SD大鼠随机分为正常组、模型组、中药预防组和中药治疗组,除正常组外其余各组用高盐高脂高糖饮食喂养。中药预防组从造模第一天开始给予柴芪汤[5.67 g/(kg·d)];中药治疗组前8周使用蒸馏水灌胃对照,从第8周开始使用柴芪汤灌胃8周。分别于8周末和16周末检测大鼠血清血糖及胰岛素,评价胰岛素抵抗情况,同时采用ELISA法检测各组大鼠血清中s VCAM-1、s ICAM-1、TNF-α及IL-6的水平,并取大鼠腹主动脉血管检测NF-κB蛋白表达。结果:模型组大鼠血糖、胰岛素、胰岛素抵抗指数、血脂、血清s VCAM-1、s ICAM-1、TNF-α及IL-6水平、血管NF-κB蛋白表达明显高于正常组(P<0.05);使用柴芪汤干预后各项指标均明显低于模型组(P<0.05);但早期预防组的各项指标较治疗组的更具有优势。结论:柴芪汤可能通过影响血清s VCAM-1、s ICAM-1、TNF-α、IL-6的水平及NF-κB蛋白从而对MS血管损伤起到一定的干预效果,同时柴芪汤早期预防用药比治疗给药效果明显。Objective ：To observe the effect of Chaiqi decoction on vascular injury of metabolic syndrome rat models and to investigate its mechanism. Methods：The rats were randomly divided into a normal group, a model group, a prevention group and a treatment group. The rat models were established by feeding high fat, high sugar and high salt diet for 8 weeks. The prevention group was given Chaiqi decoction [ 5.67 g/（ kg ·d） ] from the beginning of modeling, and the treatment group was given Chaiqi decoction since the 8th week. The blood glucose and insulin were detected at 8th and 16^th weeks. The levels of sVCAM- 1 ,sICAM- 1 ,TNF- α and IL - 6 in serum were measured by ELISA, and the protein expressions of NF - κB in the vessel were detec ted by Western blot. Results： Compared with that of the normal group, the blood glucose, insulin, insulin resistance index, the levels of sVCAM - 1, sICAM - 1, TNF -α and IL - 6 in serum, the protein expressions of NF - κB in vessel were significantly increased in model group （ P 〈0. 05 ）. All the indexes of the prevention group and treatment group were lower than those of the model group, and the difference was statistically significant （P 〈 0.05 ）. The indexes of prevention group had more advantages than those of the treatment group. Conclusion： Chaiqi decoction has some active effects on the MS vessel injury by reducing the levels of sVCAM - 1, sICAM - 1, TNF - α and IL - 6 in serum and the protein expressions of NF - κ in vessel. Using Chaiqi decoction at early stage as a prevention can have a better result than as a treatment.

关 键 词：代谢综合征 柴芪汤 血管损伤 预防及治疗 

分 类 号：R285.5[医药卫生—中药学]