转SERCA 2a基因对急性心房颤动模型心房肌细胞L型钙通道影响的研究  

The research of L type calcium channel on atrial myocytes in SERCA 2a genetic transfection models with acute atrial fibrillation

作　　者：王凌鹏[1] 崔蕴文[2] 罗健[2] 

机构地区：[1]新疆医科大学第一附属医院内科,乌鲁木齐830000 [2]新疆医科大学第一附属医院干部综合内科

出　　处：《临床心血管病杂志》2017年第5期461-465,共5页Journal of Clinical Cardiology

摘　　要：目的:研究AAV9-SERCA 2a基因转染对急性心房颤动(房颤)动物模型心房肌细胞上L型钙通道表达的影响。方法:采用60只新西兰大白兔为实验对象,采用心包注射法转染AAV9-SERCA 2a基因;将实验对象随机分为4组:房颤组、转荧光蛋白房颤组、转SERCA 2a基因房颤组和对照组。转染后建立急性房颤模型,采用膜片钳及荧光免疫组织化学法检测心房肌细胞上L型钙通道的电流变化及其L型钙通道蛋白表达的情况。结果:转SERCA 2a基因房颤组心房肌细胞上LVDCCa1c蛋白表达明显高于房颤组(P<0.05),与对照组比较差异无统计学意义。转SERCA 2a基因房颤组心房肌细胞上L型钙通道电流密度高于房颤组,差异有统计学意义(P<0.05),与对照组比较差异无统计学意义。结论:转SERCA 2a基因治疗可减少急性房颤模型心房肌细胞钙超载,减少房颤对L型钙通道电流密度的影响,对防止心脏电重构具有积极的意义。Objective：To study the effects of AAV9 - SERCA 2a gene transfection on L type calcium channel expression of atrial myocytes in animal models with acute atrial fibrillation. Method： Sixty New Zealand white rab- bit were selected as experimental objects, the pericardium injection was used to transfect AAV9 - SERCA 2a gene. The subjects were randomly divided into 4 groups： atrial fibrillation group, AAVg-EGYP group, SERCA 2a group and control group. Acute atrial fibrillation models were established after transfection. Patch clamp and fluorescent immuaohistochemical method were used to detect L type calcium channel current changes and expression. Result： LVDCCalc protein expression on atrial myocytes was obviously higher in SERCA 2a group than that in atrial fi- brillation group （P〈0.05）, and no statistically significant difference was observed between SERCA 2a group and control group. The L type calcium channel current density was higher in SERCA 2a group than that in atrial fibril- lation group （P〈O. 05）, and no statistically significant difference was observed between SERCA 2a group and control group. Conclusion： The therapy of SERCA 2a gene transfection can reduce the calcium overload on atrial myocytes in acute atrial fibrillation models, and reduce the effect of atrial fibrillation on L type calcium channel current density, which has a positive influence to prevent cardiac electrical remodeling.

关 键 词：心房颤动 转基因 电生理 SERCA 2a 

分 类 号：R541.7[医药卫生—心血管疾病]