原花青素对玉米赤霉烯酮诱导小鼠睾丸TM4支持细胞凋亡的保护作用  

Protective effect of proanthocyanidins on the sertoli cells TM4 apoptosis of mice induced by zearalenone

作　　者：师维[1] 王光磊[1] 郭佳怡[1] 张燚[1] 杨淑华[1] 张文奎[1] 李鹏[1] 龙淼[1] 

机构地区：[1]沈阳农业大学,沈阳110866

出　　处：《黑龙江畜牧兽医》2017年第6期23-27,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31201961;31302152;31640084);中国博士后科研基金项目(2014M551125);辽宁省教育厅科学研究一般项目(L2014561)

摘　　要：为了研究原花青素对玉米赤霉烯酮诱导的小鼠睾丸支持细胞TM4凋亡的保护作用,试验采用体外培养小鼠睾丸支持细胞TM4,用MTT法分别测定原花青素(PC)、玉米赤霉烯酮(ZEA)对TM4细胞生长的影响,流式细胞术检测PC对ZEA诱导的TM4细胞凋亡的保护作用,并检测PC、ZEA引起的总超氧化物歧化酶(T-SOD)的活性变化及丙二醛(MDA)的含量变化。结果表明:PC可显著增加TM4细胞的存活率,抑制TM4细胞的凋亡,提高T-SOD活性,降低MDA含量;有效抑制ZEA诱导的TM4细胞凋亡,主要是通过抗氧化作用对经ZEA染毒的TM4细胞起到保护作用。The study was aimed to investigate the protective effect of procyanidine （PC） on apoptosis of TM4 cells induced by the zearalenone （ZEA）. The effect of the growth of TM4 cells of the PC and ZEA was determined in vitro culture of TM4 by using M＇I3＂ method. The protective effects of PC on induced apoptosis of TM4 cells induced by ZEA were detected by using flow cytometry. The activities of total supemxide dis- mutase （ T - SOD） and the content of malandialdehyde （MDA） were detected and analyzed by kit. The results showed that PC can significantly increase the survival rates of TM4 cells, and exhibit inhibitory effect of ZEA on the apoptosis of TM4 cells; PC improves the T - SOD activity, and reduces the high content of MDA in ceils induced by ZEA. These data demonstrate that PC can effectively inhibit the apoptosis of TM4 cells induced by ZEA through the antioxidant effect of ZEA exposed TM4 ceils.

关 键 词：原花青素 玉米赤霉烯酮 TM4 支持细胞 氧化损伤 细胞凋亡 

分 类 号：S859.81[农业科学—临床兽医学]