片仔癀治疗石胆酸灌胃诱导胆囊炎豚鼠模型的效果观察  

Clinical effect of Pien Tze Huang in treatment of guinea pigs with cholecystitis induced by lithocholic acid given by gavage

作　　者：任彩瑗 任锋[2] 李俊峰[2] 丁美[2] 陈煜[2] 段钟平[2] 张桓虎[1] 

机构地区：[1]山西医科大学第二临床医学院消化科,太原030001 [2]首都医科大学附属北京佑安医院肝病研究所,北京100069

出　　处：《临床肝胆病杂志》2017年第5期905-908,共4页Journal of Clinical Hepatology

基　　金：国家自然科学基金项目(81270532);北京市自然科学基金项目(7162085);首都特色临床应用研究(Z121107001012167);北京市卫生系统高层次卫生技术人才培养计划(2013-3-075);北京市医院管理局"登峰"人才培养计划(DFL20151601);北京市属医学科研院所公益发展改革试点项目(京医研2016-2)

摘　　要：目的探讨传统中药片仔癀对胆囊炎动物模型的治疗效果。方法将80只豚鼠(雌雄各半)随机分为空白对照组(10只,与模型组相同剂量的PBS灌胃),胆囊炎模型组(20只,石胆酸灌胃),片仔癀组(30只,在胆囊炎模型基础上,加用片仔癀灌胃),熊去氧胆酸(UCDA)组(20只,在胆囊炎模型基础上,加用UCDA)。观察各组豚鼠的症状、胆囊病理损伤及其肝功能[ALT、AST、TBil、DBil、IBil和总胆汁酸(TBA)]变化。计量资料2组间比较采用独立样本t检验,多组间比较采用单因素方差分析,两两比较采用LSD-t检验,P<0.05为差异有统计学意义。结果与模型组相比,片仔癀组豚鼠状态明显较好,毛发较为光亮整齐,精神状态较好,饮食较好,15 d后均未出现死亡。B超和动态血流图显示,与模型组相比,片仔癀组胆囊形态基本恢复至正常水平,胆囊矢状径[(14.92±1.36)mm vs(16.51±0.65)mm]、横状径[(21.25±1.61)mm vs(23.93±0.84)mm]和胆囊厚度[(0.75±0.049)mm vs(0.90±0.03)mm]均减小,差异均有统计学意义(P值均<0.05)。HE染色表明,片仔癀治疗可显著改善胆囊病理损伤。与模型组比较,片仔癀组ALT[(316.50±264.50)U/L vs(684.71±87.73)U/L]、AST[(88.78±16.69)U/L vs(180.2±84.40)U/L]、TBil[(0.18±0.10)μmol/L vs(1.43±0.90)μmol/L]、DBil[(0.06±0.05)μmol/L vs(0.23±0.07)μmol/L]、IBil[(0.12±0.06)μmol/L vs(1.20±0.55)μmol/L]和TBA[(42.40±40.57)μmol/L vs(271.42±148.94)μmol/L]均显著降低,差异均有统计学意义(P值均<0.05)。结论片仔癀对胆囊炎豚鼠模型的症状、胆囊病理损伤和肝功能均具有显著的改善作用。Objective To investigate the clinical effect of traditional Chinese medicine Pien Tze Huang in the treatment of guinea pigs with cholecystitis. Methods A total of 80 guinea pigs （40 males and 40 females） were randomly divided into blank control group （ 10 guinea pigs treated with PBS at the same dose as that in model group）, cholecystitis model group （20 guinea pigs treated with lithochnlic acid given by garage）, Pien Tze Huang group （30 guinea pigs treated with Pien Tze Hnang by gavage in addition to the treatment in the cholecystifis model group） , and ursodeoxycholic acid group （20 guinea pigs treated with ursodeoxycholic acid in addition to the treatment in the cholecystitis model group）. All groups were observed in terms of symptoms, pathological damage of the gallbladder, and liver function [ the levels of alanine aminotransferase （ALT） , aspartate aminotransferase （AST） , total bilirubin （ TBil ） , direct bilirubin （DBil） , indirect bilirubin （IBil）, and total bile acid （TBA） ]. The two -independent -samples t test was used for comparison of continuous data between groups; a one - way analysis of variance was used for comparison between multiple groups and the least significant difference t - test was used for com- parison between any two groups. P 〈 0.05 was considered statistically significant. Results Compared with the model group, the Pien Tze Huang group had significantly better conditions with smooth hair, a good mental state, and good food consumption, and no guinea pigs died after 15 days. As was shown by ultrasound and rheography, compared with the model group, the Pien Tze Huang group had basically normal morphology of the gallbladder and significant reductions in sagittal diameter of the gallbladder （ 14.92 ± 1.36 mm vs 16.51 ±0.65 mm, P 〈0.05 ）, transverse diameter of the gallbladder （21.25 ± 1.61 mm vs 23.93 ± 0.84 mm, P 〈 0.05 ）, and gallbladder wall thickness （ 0.75 ± 0. 049 mm vs 0.90 ± 0.03 mm, P 〈 0.05 ）. HE staining s

关 键 词：胆囊炎 片仔癀 中成药 豚鼠 治疗结果 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]