化痰祛湿活血方对非酒精性脂肪性肝炎大鼠ADPN/AMPK/ACC通路的影响  

Effect of Huatan Qushi Huoxue prescription on the ADPN/AMPK/ACC signaling pathway in rats with nonalcoholic steatohepatitis

作　　者：赵文霞[1] 闫乐[1] 邵明义[1] 张丽慧[1] 

机构地区：[1]河南中医药大学第一附属医院脾胃肝胆病科,郑州450000

出　　处：《临床肝胆病杂志》2017年第5期932-936,共5页Journal of Clinical Hepatology

基　　金：河南省中医临床学科领军人才培育项目;国家自然基金(81473651)

摘　　要：目的研究化痰祛湿活血方对非酒精性脂肪性肝炎(NASH)大鼠脂联素(ADPN)/腺苷酸活化蛋白激酶(AMPK)/乙酰辅酶A羧化酶(ACC)通路的影响。方法 72只SD大鼠随机分为空白组、模型组、对照组及高、中、低剂量组,除空白组外,其余5组以高脂饲料联合四环素腹腔注射建立NASH模型,造模第2周分别给予化痰祛湿活血方高、中、低剂量和多烯磷脂酰胆碱(易善复)胶囊对照治疗。实验结束时,观察肝匀浆游离脂肪酸(FFA)含量,ELISA及RT-q PCR法测肝组织ADPN、脂联素受体2(AdipoR2)、AMPK、ACC、肉毒碱棕榈酰转移酶1(CPT-1)的基因与蛋白表达。多组间数据比较采用方差分析,方差齐者进一步两组间比较采用LSD-t检验,方差不齐者采用Dunnett's T3法。结果与对照组比较,高剂量组能明显降低肝匀浆FFA[(229.07±46.62)μmol/gprot vs(382.75±30.93)μmol/gprot]、肝组织ACC的基因(1.66±0.04 vs 2.40±0.37)、蛋白[(512.20±60.70)pg/g vs(756.72±61.21)pg/g]表达(P值均<0.01),升高ADPN、AdipoR2、AMPK、CPT-1的基因[1.53±0.31 vs 0.75±0.11,1.38±0.33 vs 0.61±0.09,1.43±0.39 vs 0.78±0.04,1.39±0.31 vs 0.52±0.04]、蛋白[(22.23±2.75)μg/g vs(14.01±2.17)μg/g,(128.41±10.46)ng/g vs(94.62±6.88)ng/g,(1353.79±59.52)μg/g vs(1107.19±56.78)μg/g,(175.54±9.91)U/g vs(128.74±12.74)U/g]表达(P值均<0.01)。结论化痰祛湿活血方能够有效激活ADPN/AMPK/ACC信号通路,影响脂肪酸的β氧化及合成,减少肝细胞脂肪沉积。Objective To investigate the effect of Huatan Qushi Huoxue prescription on the adiponectin （ ADPN ）/adenosine monophosphate -activated protein kinase （AMPK）/acetyl -CoA carboxylase （ACC） signaling pathway in rats with nonalcoholic steatohepatitis （NASH）. Methods A total of 72 male Sprague - Dawley rats were randomly divided into blank group, model group, control group, and high - , mid- die - , and low - dose Huatan Qushi Huoxue prescription groups. All rats except those in the blank group were given high - fat diet combined with intraperitoneal injection of tetracycline to establish a rat model of NASH. At week 2 after model establishment, the Huatan Qushi Huoxue prescription groups were treated with high - , middle - , and low - dose Huatan Qushi Huoxue prescription, and polyene phosphati- dylcholine （Yishanfu） capsules were given as control. At the end of the experiment, the content of free fatty acid （FFA） in liver homoge- nate was measured, and ELISA and RT - qPCR were used to measure the protein and mRNA expression of ADPN, adiponectin receptor - 2 （AdipoR2） , AMPK, ACC, and carnitine palmitoyltransferase - 1 （ CPT - 1 ） in liver tissue. An analysis of variance was used for compari- son of data between multiple groups ; the least significant difference t - test was used for comparison of data with homogeneity of variance be- tween two groups, and the Dunnett＇s T3 method was used for comparison of data with heterogeneity of variance. Results Compared with that control group, the high -dose group had significant reductions in FFA level in liver homogenate （229.07 ±46.62 μmol/gprot vs 382.75 ±30.93 μ mol/gprot, P 〈 0.01 ） and mRNA and protein expression of ACC in liver tissue （ mRNA ： 1.66 ± 0.04 vs 2.40 ± 0.37, P 〈 0.01 ; protein： 512.20 ±60.70 pg/g vs 756.72 ±61.21 pg/g, P 〈0.01 ）, as well as significant increases in the mRNA and protein expression of ADPN （mRNA： 1.53 ±0.31 vs 0.75 ± 0. 11, P 〈 0.01 ; protein： 22.23 ± 2.75 μg/g vs 14.01

关 键 词：非酒精性脂肪性肝炎 化痰祛湿活血方 脂联素 腺苷酸活化蛋白激酶 乙酰Co A羧化酶 

分 类 号：R285.5[医药卫生—中药学]