肾素基因MboI多态性与环境因素交互作用对原发性高血压的影响  

The interaction effect of renin gene MboI polymorphism and environmental factors in essential hypertension

作　　者：张国平[1] 何宁[2] 丛明宇[2] 张可勇[2] 李静平[2] 赵景波[1] 邱长春[2,3] 

机构地区：[1]哈尔滨医科大学公共卫生学院流行病教研室,黑龙江哈尔滨150081 [2]齐齐哈尔医学院医药科学研究院,黑龙江齐齐哈尔161006 [3]中国医学科学院基础学院医学分子生物学国家实验室,北京100730

出　　处：《中华疾病控制杂志》2017年第5期473-477,491,共6页Chinese Journal of Disease Control & Prevention

基　　金：黑龙江省自然科学基金项目(D201235)

摘　　要：目的探讨肾素基因MboI多态性与环境因素交互作用对原发性高血压的影响。方法采用病例对照研究,高血压病例(1 533例)来源于黑龙江省兰西县农村现场调查,对照(1 557例)来自于同一现场调查的非高血压患者。肾素基因MboI的多态性的检测,主要采用聚合酶链式反应的限制性片段长度多态性(olymerase chain reaction-restriction fragment length polymorphism,PCR-RFLPP)的方法。为确定基因分型的准确性,随机抽取10%样本的PCR产物进行直接测序。结果肾素基因MboI多态性与性别、高血压家族史以及高密度脂蛋白(high density lipoprotein,HDL)之间存在正相乘模型交互作用,ORi(odd rate of interaction)分别为1.36,1.12,1.03;与年龄、腰围(abdomen circumference,AC)、体质指数(body mass index,BMI)以及甘油三酯(triglyceride,TG)之间存在负相乘模型交互作用,ORi分别为0.82,0.47,0.46,0.70。结论男性、有高血压家族史、HDL升高,伴有MboI位点的突变时,均增加高血压的发病风险;年龄、腰围、BMI以及TG水平升高,伴有MboI位点的突变时,均降低高血压的发病风险。Objective To explore interation effect of REN MboI polymorphism and environmental factors on essential hypertension.Methods A case-control study was performed in this study.Subjects （case：1 533,control：1 557）were from a field survey in the rural of Lanxi county of Heilongjiang province.The polymorphism was mainly genotyped by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）.In addition,in order to make sure the accuracy of PCR-RFLP,10% PCR products were randomly selected to be sequenced.Results There were positive muhiplication model of interaction between MboI polymorphism and sex,family history of hypertension,high density lipoprotein （HDL）,the odds ratio of interaction （ORi） were 1.36,1.12 and 1.03,respectively;There were negative multiplication model of interaction between MboI polymorphism and age,abdomen circumference（AC）,body mass index（BMI）,triglyceride （TG）,with ORi of 0.82,0.47,0.46 and 0.70 respectively.Conclusions When nale or having family history of hypertension or high level of HDL is accompanied with mutation of MboI locus,the risk for hypertension will increase;when high level of age or AC or BMI or TG is accompanied with mutation of MboI locus,the risk for hypertension will decline.

关 键 词：肾素 基因 交互分析 高血压 

分 类 号：R544.1[医药卫生—心血管疾病]