原因不明复发性流产患者血清IL-33水平与T_H1、T_H2、T_H17及Treg细胞间的关系  

Serum IL-33 level and its correlation with T_H1,T_H2,T_H17 and Treg cells in patients with unexplained recurrent spontaneous abortion

作　　者：费倩倩 丁家怡[1] 施蔚红 金华[1] 谭小方[1] 陈小波[2] 

机构地区：[1]南通市妇幼保健院生殖医学中心,江苏南通226000 [2]南通市妇幼保健院产前诊断中心,江苏南通226000

出　　处：《临床检验杂志》2017年第4期281-284,共4页Chinese Journal of Clinical Laboratory Science

基　　金：南通市科技计划项目(HS2014012)

摘　　要：目的探讨IL-33和T_H1、T_H2、T_H17及调节性T细胞(Treg细胞)在原因不明复发性流产(URSA)患者中的水平及其相关性。方法收集46例URSA患者及40例健康人对照者,流式细胞术检测外周血T_H1、T_H2、T_H17及Treg细胞比例;ELISA检测血清IL-33浓度。结果与健康人对照组相比,URSA患者血清IL-33浓度显著下降,此外,外周血T_H1和T_H17细胞比例更高,而T_H2和Treg细胞则低于健康人对照组(P<0.05)。IL-33浓度与T_H1和T_H17细胞比例均显著负相关,而与T_H2细胞比例呈正相关性,但与Treg细胞间无显著相关性(P>0.05)。结论 URSA患者外周血IL-33浓度显著下调,并与T_H1、T_H2和T_H17等免疫细胞比例相关,提示在URSA患者中IL-33可能与T_H1、T_H2和T_H17存在关联。Objective To investigate the serum IL-33 level and its association with TH1,TH2,TH17 and Treg cells in patients with unexplained recurrent spontaneous abortion（ URSA）. Methods Forty-six URSA patients and 40 healthy controls were enrolled. The proportions of TH1,TH2,TH17 and Treg cells in peripheral blood samples were determined by flow cytometry,and serum IL-33 levels by ELISA. Results The levels of serum IL-33 in URSA patients were significantly lower than that in healthy controls. The proportions of TH2 and Treg cells in URSA patients were significantly lower than that in healthy controls（ P 〈 0. 05）,while the proportions of TH1 and TH17 cells in URSA patients were significantly higher than that in healthy controls. Serum IL-33 levels were negatively correlated with the proportions of TH1 and TH17 cells,and positively with that of TH2 cells,while no correlation with Treg cells. Conclusion Serum IL-33 levels decrease significantly in URSA patients,and are correlated with the proportions of TH1,TH2 and TH17 cells,indicating that IL-33 may be associated with TH1,TH2 and TH17 cells in URSA patients.

关 键 词：原因不明复发性流产 白介素33 辅助T细胞 调节性T细胞 

分 类 号：R446.6[医药卫生—诊断学]