半枝莲多糖对胃癌细胞SGC-7901的细胞活性影响  

Influence of Scutellaria Barbata Polysaccharide on the Cell Activity of Gastric Carcinoma Cell SGC-7901

作　　者：高山[1] 宋高臣[1] 许晓义[1] 

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011

出　　处：《中外医学研究》2017年第15期1-2,共2页CHINESE AND FOREIGN MEDICAL RESEARCH

基　　金：黑龙江省中医药中青年科技攻关项目(项目编号:2QG-056);牡丹江市科学技术计划项目(项目编号:Z2015s0028)

摘　　要：目的:研究半枝莲多糖(scutellaria barbata polysaccharides,SBP)对胃癌细胞SGC-7901细胞活性的影响。方法:利用不同质量浓度半枝莲多糖处理SGC-7901胃癌细胞,对SBP处理后的SGC-7901进行细胞计数,利用MTT法检测半枝莲多糖对胃癌细胞SGC-7901的细胞活性的影响;采用荧光定量RT-PCR对SGC-7901细胞中的野生型P53基因的表达进行检测。结果:经SBP处理后胃癌细胞SGC-7901的细胞数明显减少,SBP对SGC-7901的细胞活性的抑制呈剂量依赖性,其中20μg/ml SBP组的活性抑制率最高,野生型P53基因表达量随SBP质量浓度升高而增加,10、20μg/ml SBP组P53基因的表达量高于阴性对照组,差异有统计学意义(P<0.01)。结论:半枝莲多糖通过上调抑癌基因P53以抑制胃癌细胞SGC-7901的生长活性。Obejective： To study the effect of SBP（scutellaria barbata polysaccharides） on SGC-7901 gastric cancer cellular activity.Method： SGC- 7901 gastric carcinoma cell was handled with SBP in different mass concentration, the cellular activity that SGC-7901 under the treatment of SBP was observed by MTr, the expression of wt-P53 gene was detected in SGC-7901 including which cultured by SBP.Result： The count of SGC-7901 which had been handled by SBP reduced significantly, the depression of cellular activity had dose-dependent, the most obvious depressive activity was in the 20 μg/ml SBP group, the level of wt- P53 expression in SBP cultured SGC-7901 cell group elevated with the increase of mass concentration of SBP.The level of P53 expression in the 10 and 20μg/ml SBP group were higher than the negative control, the differences were statistically significant（P〈0.01）.Conclusion： SBP depresses the growth of SGC-7901 gastric tumor cell by upregnlating P53 gene.

关 键 词：半枝莲多糖 胃癌 增殖抑制 基因表达调控 细胞培养 

分 类 号：R285[医药卫生—中药学]