板蓝根提取物体外抗呼吸道合胞病毒机制研究  

Effect of Active Extracts from Radix Isatidis against Respiratory Syncytial Virus in Vitro

作　　者：张李唯 何立巍[2] 张军峰 姜淼[4] 佟书娟 陈建平 王任[1] 董伟 詹瑧 

机构地区：[1]南京中医药大学研究生院,江苏南京210023 [2]南京中医药大学药学院,江苏南京210023 [3]南京中医药大学医学与生命科学学院,江苏南京210023 [4]南京中医药大学基础医学院,江苏南京210023

出　　处：《辽宁中医杂志》2017年第5期1007-1011,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81473316);江苏省普通高校研究生科研创新计划(CXLX12-0581);江苏高校优势学科建设工程基金(PAPD);江苏省2015年度研究生教育教学改革研究与实践课题(JGLX15_122)

摘　　要：目的:研究板蓝根活性提取物体外抗呼吸道合胞病毒(RSV)的作用。方法:MTT法检测人喉癌上皮细胞(hep-2)中板蓝根活性提取物对RSV的抑制作用。荧光定量RT-PCR检测药物对RSV非结构蛋白NS1和L蛋白RNA合成的影响。结果:MTT结果显示药物治疗组和预防组在0.25~1 mg/m L浓度时均表现出抑制RSV的作用,其中0.5 mg/m L药物对RSV的抑制率分别是34.2%和52.2%,药物没有显示出直接杀伤和抑制RSV的效应。荧光定量RT-PCR检测表明药物作用1 h,RSV的NS1和L的RNA水平均显著低于病毒组(P<0.05)。结论:RSV感染hep-2过程中,板蓝根活性提取物具有预防和早期抗病毒复制的效应,但未显示出直接杀伤RSV的功能。Objective ： To study the effect of active extracts from radix isatidis against RSV （ respiratory syncytial virus） in vitro. Methods ： We observed the anti - RSV ability of the extracts from radix isatidis in human larynx carcinoma epithelial ceils （ hep - 2） by MrIT method. In order to explore the antiviral mechanism of radix isatidis extracts, RNA synthesis of nonstructural 1 （NS1） protein and L protein in RSV were detected by real - time fluorescent quantitative RT - PCR. Results ： Our study showed that in the concentration of 0.25,0.5,1 mg/mL, radix isatidis extracts had obvious anti - RSV effect in hep - 2 cells for both drug therapy group and preventive treatment group. Especially in the concentration of 0.5 mg/mL, extracts showed distinct antiviral efficacy in drug therapy group with 34.2% viral inhibition rate and in drug preventive group with 52.2% viral inhibition rate. When the drug and RSV were mixed together directly at 4℃ for 2 hours,no obvious antiviral effect was found in the present study. 1 hour after the drug was used on the RSV - infected cells, the result of real - time fluorescent quantitative RT - PCR indicated that RNA level of NS1 and L of RSV in drug treatment group were significantly lower than those in virus group （ P 〈 0. 05 ）. Conclusion ： Our study indicated that active extracts from radix isatidis played a preventive role on hep - 2 in the course of RSV infection. The drug could also inhibit replication of RSV at the early stage, but did not show the effect of directly killing RSV.

关 键 词：板蓝根 呼吸道合胞病毒 人喉癌上皮细胞 

分 类 号：R285.6[医药卫生—中药学]