化瘀解毒方对过表达PDK1和Akt人胃癌SGC-7901细胞增殖的影响  

Effect of Huayu Jiedu Formula on Proliferation of Human Gastric Cancer SGC-7901 Cell Line PDK1 and Akt

作　　者：张俊萍[1] 赵一[1] 梁瑞峰[1] 赵国岑[1] 魏征[1] 

机构地区：[1]河南省中医药研究院附属医院,河南郑州450004

出　　处：《辽宁中医杂志》2017年第5期1036-1039,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金;青年基金项目(81403267);国家中医药管理局全国名老中医(赵国岑)传承工作室项目(国中医人教发[2013]47号)

摘　　要：目的:利用细胞瞬时转染技术,探讨过表达PDK1和Akt后化瘀解毒方对人胃癌SGC-7901细胞增殖的抑制作用。方法:采用Elisa检测化瘀解毒方提取物对PDK1激酶活力的影响,先后将SGC-7901细胞瞬时转染PDK1质粒和Akt质粒,采用Western blot检测PI3K/Akt通路关键分子p-Akt,Akt活性变化;MTT法检测化瘀解毒方提取物对过表达PDK1或者Akt细胞增殖抑制作用。结果:化瘀解毒方提取物剂量依赖性的抑制PDK1蛋白激酶活性。免疫印迹结果显示过表达PDK1或者Akt可以阻止化瘀解毒方提取物或者LY294002抑制SGC-7901细胞中Akt蛋白的磷酸化。MTT结果显示,化瘀解毒方提取物剂量依赖性的抑制转染空质粒组中SGC-7901细胞增殖。结论:化瘀解毒方通过PI3K/Akt通路抑制胃癌增殖作用。Objective：To investigate the inhibitory effect of PDKI and Akt on proliferation of human gastric cancer cell line SGC -7901 by transient transfection technique. Methods：The effects of Huayu Jiedu Formula on the activity of PDK1 kinase were detected by ELISA. The SGC - 7901 cells were transiently transfection PDK1 plasmid and Akt plasmid. Western blot was used to detect the PI3K/Akt pathway key molecules Akt and Akt activity. MTT assay was used to detect the Huayu Jiedu Formula on expressions of PDK1 and Akt inhibition of cell proliferation. Results：The dose -dependent inhibition of PDK1 protein kinase activity was inhibited by the extract of removing blood stasis and toxin removing method. Western blot results showed that PDK1 or Akt could inhibit the phosphorylation of Akt protein in SGC - 7901 cells by inhibiting the extract of Huayu Jiedu Formula or LY294002. MTT results showed that the dose - dependent inhibition of SGC - 7901 cell proliferation was inhibited by Huayu Jiedu Formula. Conclusion：Huayu Jiedu Formula inhibits the proliferation of gastric cancer through PI3K/Akt pathway.

关 键 词：化瘀解毒方 胃癌 PI3K/AKT通路 基因转染 

分 类 号：R285.5[医药卫生—中药学]