不同年龄侧柏内生真菌多样性研究  

Endophytic Fungi Diversity of Platycladus orientalis in Different Age

作　　者：王京[1] 吉力尔鬼 刘建军[3,4] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100 [2]布拖县林业局,四川凉山615000 [3]西北农林科技大学风景园林艺术学院,陕西杨凌712100 [4]宁夏贺兰山森林生态系统定位站,宁夏银川750000

出　　处：《西南林业大学学报（自然科学）》2017年第3期113-117,共5页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家林业局林业公益性行业科研专项(201404302)资助

摘　　要：采用组织分离法,并结合分子生物学数据与形态特征对陕西省黄陵县黄帝陵不同年龄阶段侧柏群分离的内生真菌菌株进行鉴定。结果显示:不同年龄阶段侧柏鳞叶内生真菌的分离率,树龄<100 a的侧柏分离率最高,为53.2%,100 a≤树龄<600 a和≥600 a的侧柏分离率较低,分别为24.6%和24.8%;不同年龄阶段均表现出较丰富的多样性,其指数在2.4~2.7之间。不同年龄阶段相似性有一定差异,100 a≤树龄<600 a和≥600 a的相似性系数最高,为0.743;树龄<100 a与100 a≤树龄<600 a和树龄≥600 a之间的相似性较低,分别为0.526和0.541。侧柏鳞叶优势菌群基本保持不变,Dimorphospora、Epicoccum、Alternaria和Paecilomyces均为其各个年龄阶段的优势菌群。The endophytic fungal strains isolated from different ages of Platycladus orientalis in Huangdi Mau- soleum of Huangling County in Shaanxi Province were identified by tissue isolation method combined with molecular biological data and morphological characteristics. The endophytic fungi showed the highest isolation rate of 53.2% at the age less than 100 years. While the age greater than or equal to 100 years with the age less than 600 years, and the age greater than or equal to 600 years, the separation rate was lower, 24.6% and 24. 8% respectively. The endophytic fungi of different age stages showed rich diversity, and the index was between 2.4 and 2.7. The similar- ity of endophytic fungi population was different in different age stages. The similarity coefficient of endophytic fungi between the age greater than or equal to 100 years with less than 600 years and greater than or equal to 600 years was 0. 743 ; The similarity between the age less than 100 years and the age greater than or equal to 100 years with less than 600 years was 0. 526; The similarity between the age less than 100 years and the age greater than or equal to 600 years was 0. 541. Dominant species of Dimorphospora, Epicoccum, Alternaria, and Paecilomyces were the predominant microflora of all age groups.

关 键 词：侧柏 内生真菌 树龄 生物多样性 鳞叶 

分 类 号：S763.15[农业科学—森林保护学]