NKT细胞激活剂α-Galcer对小鼠红白血病的抑制作用  

Inhibitory effect of NKT cell stimulatorα-Galcer on development of erythroleukemia

作　　者：孙贺军 王佳骥 王姗 刘遄 陈光 左蓉洁 闫振宇 郑全辉 

机构地区：[1]华北理工大学基础医学院,河北唐山063000 [2]华北理工大学附属医院血液病科,河北唐山063000

出　　处：《华北理工大学学报（医学版）》2017年第3期169-172,共4页Journal of North China University of Science and Technology：Health Sciences Edition

基　　金：华北理工大学大学生创新创业训练计划项目(编号:X2016092);国家自然科学基金项目(编号:81373111)

摘　　要：(1)目的采用尾静脉注射制备小鼠红白血病模型,探讨α-半乳糖苷神经酰胺(α-Galcer)对红白血病发生和发展的影响。(2)方法体外培养小鼠红白血病细胞系FBL-3,经尾静脉注射接种C57BL/6小鼠。将FBL-3接种小鼠进一步分为α-Galcer处理组和溶剂对照处理组,于FBL-3接种当天分别注射α-Galcer和溶剂DMSO.观察不同处理组FBL-3细胞接种小鼠总体变化,并于FBL-3接种后第8周处死小鼠,观察外周血血象变化和肿瘤结节形成情况。(3)结果与正常小鼠相比,FBL-3细胞接种小鼠实验期间皮肤感染增加。外周血有核红细胞增加,并且出现明显的实体器官肿瘤结节,而α-Galcer处理显著抑制实体器官肿瘤结节的形成。(4)结论通过FBL-3细胞尾静脉注射初步建立红白血病肿瘤小鼠模型,α-Galcer处理对小鼠白血病的发展发挥抑制作用。Objective To prepare erythroleukemia mouse model through tail vein injection of FBL-3 cell and investigate the effect of a- Galcer on the occurrence and development of erythroleukemia.Methods FBL-3 erythroleukemia cells were cultured in vitro and C57BL/6 erythroleukemia mouse models were prepared by tail vein injection of FBL-3 cells. The FBL-3 injected mice were further divided into two groups by injecting a- Galcerand solvent DMSO respectively on the date of FBL- 3 injection. The overall changes of FBL-3 injected mice in each group were observed during this experiment. 8 weeks after FBL-3 injection, the FBL-3 injected mice were killed and peripheral blood and tumor nodules formation were detected. Results Compared with normal C57BL/6 mice, the FBL-3 injected mice demonstrated obvious skin infection, increased nucleated red blood cells and apparent tumor nodules formation, whilea- Galcer treatment significantly inhibited the formation of tumor nodules on solid organs. Conclusion Erythroleukemia mouse models were prepared preliminarily by tail intravenous injection of FBL-3 cells, and a- Galcer treatment exhibited an inhibitory effect on the development of leukemia in mice.

关 键 词：α-半乳糖苷神经酰胺 红白血病 肿瘤小鼠模型 

分 类 号：Q784[生物学—分子生物学]