蒲地灌肠液中总多糖含量测定与方法学验证  

The methodology validation and content assay of total polysaccharides in Pudi Enema

作　　者：王艺红[1] 陈锦珊[1] 许彩燕[2] 郑绍忠[1] 

机构地区：[1]解放军175医院/厦门大学附属东南医院制剂科,福建漳州363000 [2]解放军175医院/厦门大学附属东南医院药学科,福建漳州363000

出　　处：《药学实践杂志》2017年第3期248-251,共4页Journal of Pharmaceutical Practice

摘　　要：目的建立蒲地灌肠液中总多糖的含量测定方法。方法采用苯酚-硫酸法显色,紫外分光光度法在488.8nm波长处测定总多糖的含量。结果总多糖在0~22.635 mg/L范围内呈良好线性关系;其回归方程为Y=0.062 06 X-0.003 34(r=0.999 8),回收率为98.36%,RSD为2.34%(n=6)。结论该方法灵敏、快速、准确、可靠,可用于蒲地灌肠液中总多糖含量的测定。Objective To establish the assay method for the total polysaccharide in Pudi Enema.Methods Phenol-sulfuric acid method was used for chromogenic reaction.The content of total polysaccharide was measured by UV spectrophotometry at 488.8 nm.Results The total polysaccharides calibration curve was at the range of 0-22.635 mg/L, with regression function being Y=0.062 06 X-0.003 34（r=0.999 8）.The recovery of calycosin was 98.36%（RSD=2.34%）.Conclusion This method is sensitive,rapid,accurate and reliable.It can be used to assay the content of total polysaccharide in Pudi Enema.

关 键 词：蒲地灌肠液 总多糖 苯酚-硫酸法 紫外分光光度法 含量测定 

分 类 号：R927[医药卫生—药学]