高效液相色谱法测定降脂护肝胶囊中葛根素的含量  

Determination of pueratin in Jiangzhi Hugan capsule by HPLC

作　　者：郑巍[1] 倪东杰[1] 韦庆[1] 向会[1] 胡兰兰[1] 孙亮[1] 

机构地区：[1]解放军98医院,浙江湖州313000

出　　处：《药学实践杂志》2017年第3期259-261,共3页Journal of Pharmaceutical Practice

摘　　要：目的建立高效液相色谱法测定降脂护肝胶囊中葛根素的含量。方法色谱柱为Waters Symmetry C_(18)柱(4.6mm×250mm,5μm),流动相为乙腈-1%甲酸(11∶89),检测波长250nm,流速1.0ml/min,柱温30℃,进样量10μl。结果葛根素在2~40μg/ml范围内(r=0.999 8)呈良好的线性关系,精密度实验中RSD均小于2%,加样回收率在98%~103%。结论该测定方法简便、准确,分离效果好,可用于降脂护肝胶囊的质量控制。Objective To develop a HPLC method for determination of pueratin.Methods The separation was carried out on a Waters Symmetry C18 column（4.6 mm×250 mm, 5 μm）, the mobile phase was composed of acetonitrile and 1% formic acid（11∶89）, the detection wavelength was set at 250 nm, the flow rate was 1.0 ml/min, the column temperature was 30 ℃ and the injection volume was 10 μl.Results The linearity was obtained over 2-40 μg/ml （r=0.999 8） for pueratin.The RSD of precision were less than 2%.The average recovery was between 98% and 103%.Conclusion This HPLC method was simple, accuracy and suitable for the quality control of Jiangzhi Hugan capsule.

关 键 词：高效液相色谱法 降脂护肝胶囊 葛根素 含量测定 

分 类 号：R927[医药卫生—药学]