压力超负荷致心肌肥厚形成过程中收缩压和心重指数的变化  

Systolic pressure and heart heavy index changes in whole process of overloaded heart hypertrophy

作　　者：梁悦[1] 张光涛[2] 汤晓萌[1] 王迎春[1] 刘玉琴[1] 史君[3] 

机构地区：[1]北京大学深圳医院药学部,广东深圳518030 [2]北京大学深圳医院肝胆外科,广东深圳518030 [3]内蒙古医科大学基础医学院生理学教研室,内蒙古呼和浩特010059

出　　处：《中国医药导报》2017年第14期16-19,共4页China Medical Herald

基　　金：内蒙古自治区自然科学基金项目(2013MS1126);内蒙古自治区科技计划项目(kjt15sf10)

摘　　要：目的探讨从心血管压力负荷增加到心肌肥厚形成的全过程中收缩压和心重指数的变化。方法选用健康雄性Wistar大鼠80只,随机分为假手术组与腹主动脉缩窄组,每组各40只。两组大鼠分别随机分配到4个时间点(术后3 d、1周、2周和4周),各10只。参照腹主动脉缩窄术制作压力负荷性心肌肥厚大鼠模型,大鼠收缩压采用无创尾动脉血压测定分析系统进行测量,电子天平称量左心室重和体重,随后计算心重指数(LVW/BW)。结果与假手术组相比较,腹主动脉缩窄组术后3 d时,即表现出收缩压升高,差异有统计学意义(P<0.05);在术后1周,收缩压继续升高,心重指数开始增加,差异有统计学意义(P<0.05);术后2、4周,收缩压和心重指数继续增加,差异有统计学意义(P<0.05)。结论大鼠造模1周时开始出现心肌肥厚,模型在4周时比较稳定,利用腹主动脉缩窄术制作压力超负荷心肌肥厚大鼠模型成功。Objective To discuss the change of systolic pressure and heart weight index （HWI） in the process of cardiovascular pressure overload to myocardial hypertrophy. Methods 80 healthy male Wistar rats were randomly divided into two groups, sham operation group and abdominal aorta group. Each group was randomly separated to 4 time points （3 d, 1 week, 2 weeks, 4 weeks after surgery）. Hypertrophy rats model was made by transverse aortic constriction （TAC）, systolic blood pressure was measured by noninvasive tail arterial blood pressure measurement and analysis system. The weight of the left ventricular and body was measured by an electronic balance, and the HWI was then calculated. Results Compared with sham operation group, 3 d after TAC surgery, systolic pressure of abdominal aorta group rose, the difference was statistically significant （P 〈 0.05）; 1 week after TAC surgery, systolic pressure of abdominal aorta group rose, HWI increased, the differences were statistically significant （P 〈 0.05）; 2, 4 weeks after TAC surgery, systolic pressure of abdominal aorta group rose, HWI increased, the differences were statistically significant （P 〈 0.05）. Conclusion Rat myocardial hypertrophy model is formed gradually after 1 week and the model is stable in the fourth week. By abdominal TAC, the pressure overload was successfully produced and the myocardial hypertrophy can be induced successfully.

关 键 词：心肌肥厚 腹主动脉缩窄术 收缩压 心重指数 

分 类 号：R542.2[医药卫生—心血管疾病] R332[医药卫生—内科学]