磺酸化-β-环糊精作为手性选择剂分离4种碱性药物对映体  

Enatiomeric separation of four chiral drugs with hydroxypropyl-β-cyclodextrin as a chiral selector

作　　者：胡迎莉 王宇[1] 粟媛[1] 自汝婕 袁杰[1] 郭兴杰[1] 姜珍[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2017年第5期396-399,共4页Journal of Shenyang Pharmaceutical University

基　　金：2015年沈阳药科大学大学生创新创业训练项目(国家级);国家自然科学基金面上项目(81273476)

摘　　要：目的建立高效毛细管电泳法分离苯丙哌林、益康唑、酚苄明和美西律共4种手性药物对映体。方法采用未涂层的熔融毛细管柱,以磺酸化-β-环糊精(sulfated-β-cyclodextrin,S-β-CD)为手性选择剂,背景电解质选用磷酸盐缓冲液,分离电压为20 k V。分别考察了手性选择剂S-β-CD的质量浓度、背景电解质p H值、缓冲溶液浓度和温度对对映体分离的影响,优化了分离条件。结果在最佳条件下,4种手性药物对映体均得到完全分离,最佳分离度(R)分别为6.4、4.2、14.7和3.1。结论 S-β-CD对4种碱性药物均具有较高的对映体选择性。Objective Establish a high performance capillary electrophoresis method to separate four basicity drug enantiomers, benproperine, econazole, phenoxybenzamine and mexiletine. Methods The separation of enantiomers was performed in an uncoated fused capillary column, with sulfated-beta-cyclodextrin（S-β-CD） as the chiral selection agent, and phosphate buffer solution as the background electrolyte. The separation voltage was 20 kV. The influences of S-β-CD concentration, buffer pH, buffer solution concentration and temperature on the separation were investigated, and optimized. Results Under the optimal condition, four kinds of racernic drug are completely separated, and the best resolution （ R ） was 6. 7,4. 2, 14. 8 and 3. 1 respectively. Conclusions Good enantio-selectivity was obtained with S-β-CD for four kinds of basic drugs.

关 键 词：毛细管电泳法 分离对映体 磺酸化-β-环糊精 

分 类 号：R917[医药卫生—药物分析学]