复合性状转基因作物及产品检测与溯源技术研究进展  

Research Progress on Detection and Traceability Technology of Stacked Transgenic Plants and Their Products

作　　者：李鑫星[1] 周婧[1] 许文涛[2] 焦伟华[3] 刘恒一[1] 张领先[1] 

机构地区：[1]中国农业大学信息与电气工程学院,北京100083 [2]中国农业大学食品科学与营养工程学院,北京100083 [3]山东财经大学农业与农村经济研究中心,济南250014

出　　处：《农业机械学报》2017年第5期117-127,共11页Transactions of the Chinese Society for Agricultural Machinery

基　　金：转基因生物新品种培育重大专项(2014ZX0801202B)

摘　　要：随着农业育种水平的进步与发展,现有的单一性状转基因作物已经不能满足农业的需求,聚合多种优良性状的复合性状转基因作物已成为转基因作物发展的趋势。在总结和整理国内外研究文献的基础上,阐述了复合性状转基因作物的检测方法。基于蛋白质的检测技术,包括免疫试纸检测技术和ELISA检测技术。基于核酸的检测技术,包括PCR检测技术、基因芯片技术、等温扩增技术和第二代测序技术。除此之外,还阐述了转基因作物及产品的溯源技术。综述结果表明,转基因检测技术逐渐向高通量、高准确度和高灵敏度方向发展,同时一些新的检测技术也开始出现并发展;构建溯源系统需要将计算机技术与信息技术相结合;为了综合多种技术的优点,溯源系统的开发势必需要将多种信息技术结合起来使用。As the development of breeding technology, the transgenic crops of single-character were not able to meet the demands of modern agriculture, and the stacked transgenic plants that combine different characters have gradually raised the public＇s attention. Based on the literature review, some detection methods of stacked transgenic plants were introduced. The first detection technology was based on the protein, including lateral flow strip detection technique and ELISA detection technology; the second one was based on nucleic acid detection technology, including PCR detection technology, gene-chip technology, isothermal amplification technology and next-generation sequencing technology. In addition, the traceability technology of genetically modified crops and their products was also introduced. The results indicated that the transgenic detection technology was gradually developing to high throughput, high accuracy and high sensitivity and some new detection technologies had appeared and developed; the integration of computer technology and information technology would contribute to the traceability system building ; the developing trend of the traceability technology would inevitably need to combine a variety of information technologies in order to integrate the advantages of them.

关 键 词：复合性状转基因作物 安全评价 检测 溯源 

分 类 号：X826[环境科学与工程—环境工程] Q788[生物学—分子生物学]