金沙柚及其近缘种质基于SSR分子标记的遗传多样性分析  

Genetic Diversity of Jinsha Pomelo and Its Closely Related Germplasm Assessed by SSR Molecular Markers

作　　者：刘召亮[1] 万水林[1] 闫承璞[1] 胡钟东[1] 何新华[2] 曾平章 黄其高 

机构地区：[1]江西省农业科学院园艺研究所,南昌330200 [2]广西大学农学院,南宁530004 [3]江西省吉安市果业局,江西吉安343000 [4]江西省吉水县果业蔬菜局,江西吉水331600

出　　处：《中国南方果树》2017年第3期1-4,共4页South China Fruits

基　　金：国家自然科学基金(31501718;31460508);江西省农业科学院博士启动基金(20142CBS008);江西省对外科技合作计划(20141BDH80005);江西省农科院创新基金(2013CJJ009);国家现代农业柑桔产业技术体系广西创新团队南宁试验站项目(nycytxgxcxtd-05-08)资助

摘　　要：应用SSR分子标记对4份不同来源的金沙柚种质及4份金沙柚近缘种质的遗传多样性进行分析。结果表明,通过筛选的26对引物,共检测到等位基因92个,多态性为53.68%;每对引物可检测到的等位基因数目为2~8个不等,平均每对引物扩增出3.54个等位基因。8份种质的亲缘关系较近,相似系数为0.729~0.924。在相似系数为0.738时,8份种质可以被划分为金兰柚组和沙田柚组两个组。SSR molecular markers were used to analyze the genetic diversity of 8 Jinsha pomelo germplasm and its closely related varieties from main planting area of Jiangxi province. A total of 92 alleles were detected from 26 pairs of selected primers with 53.68% of polymorphism; The alleles detected per locus were ranged from 2 to 8. On average, 3.54 alleles were amplified from per pair of primers. The similarity coefficient of 8 varieties is 0. 729-0. 924. According to the genetic similarities based on UPGMA method, all materials were divided into two groups of Jinlan pomelo and Shatian pomelo at the similarity coefficient of 0. 738.

关 键 词：金沙柚 近缘种 SSR分子标记 

分 类 号：S666.3[农业科学—果树学]