氢氧化铝与黄连碱复合佐剂对甲肝疫苗诱导小鼠体液免疫影响  

Effects of compound adjuvant of aluminium hydroxide and coptisine on humoral immune response induced by hepatitis A vaccine in mice

作　　者：李思瑾[1,2] 胡凝珠[1,2] 李彦涵[1,2] 马静[3] 何慧[1,2] 乌美妮[1,2] 胡云章[1,2] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,云南昆明650118 [2]云南省虫媒传染病防控研究重点实验室 [3]昆明医科大学

出　　处：《中国公共卫生》2017年第5期734-736,共3页Chinese Journal of Public Health

基　　金：国家科技支撑计划项目(2014BAI01B01);国家自然科学基金(31500748)

摘　　要：目的探讨氢氧化铝与黄连碱复合佐剂对甲肝疫苗诱导小鼠体液免疫的影响。方法选取6~8周龄雌性ICR小鼠49只,随机分为空白组、单纯抗原组、单纯黄连碱佐剂组、铝佐剂组及复合佐剂低、中、高剂量组,每组7只;在免疫后第4、8、12、16周,采用间接酶联免疫吸附测定法(ELISA)检测小鼠分离血清中的抗HAV IgG抗体水平。结果在免疫后第4、8、12、16周,除空白组外,各组小鼠均能检测到抗HAV IgG抗体,且抗体水平随免疫后时间的延长均先升高而后降低;在免疫后第12周,各组小鼠血清中抗HAV IgG抗体水平达到峰值,单纯抗原组、单纯黄连碱佐剂组、铝佐剂组及复合佐剂低、中、高剂量组小鼠分别为log_(10)(2.908±0.413)、log_(10)(2.960±0.315)、log_(10)(3.460±0.295)、log_(10)(3.460±0.351)、log_(10)(3.470±0.251)、log_(10)(3.470±0.251);在免疫后第4周,复合佐剂低剂量组小鼠抗HAV IgG抗体水平均高于单纯抗原组和单纯黄连碱佐剂组(均P<0.05);在免疫后第8、12、16周,复合佐剂低、中、高剂量组小鼠抗HAV IgG抗体水平均高于单纯抗原组和单纯黄连碱佐剂组(均P<0.05);在免疫后第12周,铝佐剂组小鼠抗HAV IgG抗体水平均高于单纯抗原组和单纯黄连碱佐剂组(均P<0.05);在免疫后第16周,铝佐剂组小鼠抗HAV IgG抗体水平高于单纯抗原组(P<0.05)。结论适当配比的黄连碱和氢氧化铝复合佐剂可快速、显著提高甲肝疫苗诱导小鼠的体液免疫应答,有望成为一种新的人用疫苗佐剂。Objective To investigate the effect of the compound adjuvant composed of aluminium hydroxide （AI（ OH）3） and coptisine （COP） on humoral immune response induced by hepatitis A virus （HAV） antigen in mice. Methods Forty-nine female ICR mice of 6 - 8 weeks old were randomly divided into seven groups （7 mice in each group） ：blank control group （treated with phosphate buffered saline）, antigen control group （18 endotoxin unit （ EU HAV ）, COP group （ HAV 18 EU ＋ 50μg COP）, （ A1 （OH） 3 ） group （ 18 EU HAV ＋ 300μg Al （ OH ） 3 ）, low, moderate, and high-dose compound adjuvant groups （18EU HAV ＋ 300 μg A1 （OH）3 ＋ 25,50, and 100μg COP）. Blood samples of the mice in different groups were collected at the 4th,8th, 12th, and 16th week after the treatments for the detection of serum anti-HAV immunoglobulin G （IgG） with indirect enzyme-linked immunosorbent assay （ELISA）. Results After the immunization, serum anti-HAV IgG was detected in the mice of all groups, except for those of blank control, at 4th, gth, 12th, and 16th week of the treatment; the serum IgG level of the mice increased first and then decreased with the duration of the treatment and the level reached the highest at 12th week, with the value of log10 （2. 908 ± 0. 413 ） for antigen control,log10 （2. 960±0.315） for COP group,log10 （3. 460±0. 295） for A1 （OH）3 group,and log,0 （3. 460±0. 351 ）, log10 （ 3. 470 ± 0.251 ） and log10 （ 3. 470± 0.251 ） for low, moderate, and high-dose compound adjuvant groups, respectively. At the 4th week after the immunization, the serum anti-HAV IgG level of the mice treated with low-dose compound adjuvant was significantly higher than those in adjuvant-free antigen control group and COP group （ both P 〈 0. 05 ） ;at the 8th, 12th, and 16th week after the immunization, the serum anti-HAV IgG levels of mice treated with various doses of compound adjuvant were significantly higher than those in adjuvant-free antigen control

关 键 词：氢氧化铝 黄连碱 复合佐剂 甲肝疫苗 体液免疫 影响 

分 类 号：R392-33[医药卫生—免疫学]