清脑片对大鼠脑缺血再灌注损伤的预防作用  

Preventive Effect of Qingnao Tablet on Cerebral Ischemia-reperfusion Injury in Rats

作　　者：黎丹东 谢国旗 高延玲 苏峰 王希东 李艳 张正臣 

机构地区：[1]解放军第371医院医务处,河南新乡453000 [2]解放军第371医院脑外科,河南新乡453000 [3]解放军第371医院感染科,河南新乡453000 [4]解放军第371医院药械科,河南新乡453000

出　　处：《中国药房》2017年第16期2198-2201,共4页China Pharmacy

基　　金：河南新乡重点科技攻关项目(No.CXGG16054)

摘　　要：目的:研究清脑片对大鼠脑缺血再灌注损伤的预防作用。方法:取大鼠随机分为假手术组、模型组、脑络通胶囊组(阳性对照,0.05 g/kg)和清脑片高、中、低剂量组(1.52、0.76、0.38 g/kg),每组10只。各给药组大鼠ig相应药物,假手术组和模型组大鼠ig等体积羧甲基纤维素钠溶液,每天1次,连续5 d。末次给药1 h后,除假手术组外其余各组大鼠均采用线栓法制作脑缺血再灌注损伤模型。于缺血再灌注22 h后,进行大鼠神经功能缺失评分,观察脑组织海马区病理学变化,测定脑组织中超氧化物歧化酶(SOD)、三磷酸腺苷(ATP)、乳酸脱氢酶(LDH)、肿瘤坏死因子α(TNF-α)水平。结果:与假手术组比较,模型组大鼠出现不同程度神经功能缺失(评分降低)以及海马区神经元稀疏、排列不规则等病理改变;脑组织中ATP、SOD水平降低(P<0.01),LDH、TNF-α水平升高(P<0.01)。与模型组比较,清脑片各剂量组大鼠神经功能缺失评分升高(P<0.05),病理损伤改善;除清脑片低剂量组外,其余各给药组大鼠脑组织中各指标也显著改善(P<0.05或P<0.01)。结论:清脑片可升高脑缺血再灌注损伤模型大鼠脑组织中ATP、SOD水平,降低LDH、TNF-α水平,并明显改善大鼠病理损伤。OBJECTIVE： To study the preventive effect of Qingnao tablet on cerebral ischemia-reperfusion injury in rats. METHODS： Rats were randomly divided into sham operation group, model group, Naoluotong capsule group （positive control, 0.05 g/kg）, Qingnao tablet high-dose, medium-dose, low-dose groups （1.52, 0.76, 0.38 g/kg）, 10 in each group. Rats in all ad- ministration groups were intragastrically given relevant medicines, rats in sham operation group and model group were intragastrical- ly given equal volume of sodium carboxymethylcellulose solution, once a day, for 5 d. After 1 h of last administration, all rats were induced for cerebral ischemia-reperfusion injury model by suture-occluded method except for sham operation group. Aider 22 h of ischemia-reperfusion, neurological fimction deficit scoring was conducted; the pathological changes of the hippocampus were ob- served; superoxide dismutase （SOD）, adenosine triphosphate （ATP） , lactate dehydrogenase （LDH） , tumor necrosis factor （TNF-α） levels in brain tissue were measured. RESULTS： Compared with sham operation group, rats in model group appeared different degrees of neurological deficits （score declined）, sparse neurons, irregularly arranged in hippocampus as well as other patho- logical changes; ATP, SOD levels in brain tissue were decreased （P〈 0.01 ）, LDH, TNF-α levels were increased （P〈 0.01）. Compared with model group, neurological function deficit scores in Qingnao tablet doses groups were increased （P〈0.05）, neurological deficits were improved. Except for sham operation group, brain tissue indexes in other administration groups were significantly improved （P〈0.05 or P〈0.01）. CONCLUSIONS： Qingnao tablet can increase ATP and SOD levels in brain tissue homogenate of model rats with cerebral ischemia-reperfusion, decrease LDH and TNF-α levels, and obviously improve rats＇ cerebral ischemia-re- perfusion injury.

关 键 词：清脑片 脑缺血再灌注损伤 大鼠 预防作用 

分 类 号：R965.1[医药卫生—药理学]