黎药益智仁不同极性部位提取物对实验性肠炎模型小鼠的影响  

Effects of Extracts from Different Polar Parts of Li Medicine Alpinia oxyphylla Fruit on Model Mice with Experimental Colitis

作　　者：房磊臣[1,2] 刘嫱[1] 高新征[1] 韦祎[1] 刘一鸣[1] 黄凌[1] 

机构地区：[1]海南医学院药理教研室,海口571101 [2]北华大学附属医院生殖中心,吉林吉林132011

出　　处：《中国药房》2017年第16期2220-2223,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81360586;81473618);海南省自然科学基金资助项目(No.812186;814290);海南省中药现代化专项项目(No.ZY201332);海口市重点科技计划项目(No.2013-50)

摘　　要：目的:考察黎药益智仁4种不同极性部位提取物对实验性肠炎模型小鼠的影响。方法:将益智仁70%乙醇提取物浸膏用水分散后,依次用石油醚、三氯甲烷、乙酸乙酯和正丁醇提取得相应部位提取物。将42只小鼠随机分为造模组(36只)和空白组(6只),造模组大鼠采用2,4,6-三硝基苯磺酸法复制实验性肠炎模型。成模后,将造模小鼠随机分为模型组、阳性组(柳氮磺胺吡啶肠溶片,52 g/kg)和益智仁不同极性部位(石油醚、三氯甲烷、乙酸乙酯和正丁醇部位)提取物组(剂量均为10 g/kg,以生药计),每组6只,ig给药,每天1次,连续9 d,给药体积为0.2 m L/10 g;空白组和模型组小鼠ig生理盐水。测定小鼠体质量变化,对疾病活动指数(DAI)进行评分,测定结肠组织中髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)、丙二醛(MDA)活性,观察结肠组织病理变化并进行评分。结果:与空白组比较,模型组小鼠体质量下降百分率、DAI评分以及结肠组织中MPO、MDA活性均显著升高(P<0.05或P<0.01),结肠组织发生明显病变。与模型组比较,各给药组小鼠体质量显著回升,DAI评分显著降低,MPO活性显著降低,病理评分显著降低;阳性组、益智仁正丁醇提取物和乙酸乙酯提取物组小鼠结肠组织中SOD活性显著升高,阳性组和益智仁乙酸乙酯提取物组小鼠结肠组织中MDA活性显著降低,差异均有统计学意义(P<0.05或P<0.01)。结论:益智仁4种极性部位提取物对实验性肠炎模型小鼠均有一定改善作用,其中以乙酸乙酯部位和正丁醇部位效果较好。OBJECTIVE ： To investigate the effects of 4 polar parts extracts from Li medicine Alpinia oxyphylla fruit on model mice with experimental colitis, and screen the effective parts of A. oxyphylla fruit in the treatment of colitis. METHODS： After 70% ethanol extract dispersed by water, petroleum ether, trichloromethane, ethyl acetate and n-butanol were used in turn to obtain extractions in related parts. 42 mice were randomly divided into modeling group （36 mice） and blank group （6 mice）. Mice in mod- eling group were taken 2, 4, 6-nitrobenzne sulfonic acid method to replicate the experiment colitis. After modeling, model mice were randomly divided into model group, positive group （Sulfasalazine enteric coated tablets, 52 g/kg）, petroleum ether, trichloro- methane, ethyl acetate, n-butanol extract from A. oxyphylla fruit groups （10 g/kg, calculated by crude drug）, 6 in each group, in- tragastrically administrated once a day, for 9 d, 0.2 mL/10 g; mice in blank group and model group were intragastrically given nor- mal saline. After administration, body mass of mice was determined, disease activity index （DAI） was scored and colonic myelo- peroxidase （MPO）, superoxide dismutase （SOD）, malondialdehyde （MDA） activities in colon tissue were detected, and the colon pathological changes were observed and scored. RESULTS： Compared with blank group, decrease percentage of body mass, DAI score, MPO and MDA activities in colon tissue in model group were increased （P〈0.05 or P〈0.01） ; colon tissue was pathologi- cally changed. Compared with model group, body mass was increased, DAI score, MPO activity and pathological score in each ad- ministration groups were decreased; SOD activities in positive group, n-butanol extract from A. oxyphylla fruit group and ethyl ace- tate extract from A. oxyphylla fruit group were significantly increased, MDA activities in positive group and ethyl acetate extract from A. oxyphylla fruit group were significantly decreased, with statistical significan

关 键 词：益智仁 提取部位 实验性肠炎 小鼠 

分 类 号：R285[医药卫生—中药学]