食蟹猴腹腔注射低剂量百草枯致胰腺α-突触核蛋白的病理变化  

The Pathological Changes of α-Synuclein in Pancreas of Cynomologus Monkeys by Intraperitoneal Injection of Small Dose of Paraquat

作　　者：胡亚松 罗斌斌 陶国现 岳峰[1,2] 陈彪[1,3] 

机构地区：[1]广西医科大学转化医学研究中心长寿与老年相关疾病省部共建教育部重点实验室,南宁530000 [2]广西南宁灵康赛诺科生物科技有限公司,南宁530000 [3]首都医科大学宣武医院神经生物学研究室,北京100053

出　　处：《基因组学与应用生物学》2017年第5期1732-1736,共5页Genomics and Applied Biology

基　　金：国家高技术研究发展计划(2012AA02A514);国家重点基础研究发展计划(2011CB504101);广西区科技厅项目(1598025-31);南宁市科技局项目(20155192;20163349)共同资助

摘　　要：本研究旨在通过食蟹猴经腹腔注射低剂量百草枯来探讨胰腺α-突触核蛋白(α-synuclein,α-syn)的病理变化。由广西南宁灵康赛诺科生物科技有限公司提供的12只(8~10岁)健康雄性食蟹猴为实验动物,随机分为实验组A、B和正常对照组C,每组4只,实验组分别于给药百草枯(paraquat,PQ)5个月,2个月后安乐死(对照组每阶段2只)。利用免疫组织化学方法和免疫蛋白印记方法检测各组食蟹猴胰岛α-syn的表达特点和表达量。结果显示,α-syn免疫活性物质主要见于胰岛细胞胞浆,并且α-syn在各组食蟹猴胰岛存在差异表达,其中给药百草枯5个月后的食蟹猴胰岛α-syn表达量最高,对照组表达量最低。我们的研究表明食蟹猴腹腔注射低剂量百草枯可致胰岛α-syn表达上调,百草枯是造成胰岛腺α-syn差异表达的关键因素之一,其结论将为相关疾病的模型制作、早期诊断及干预提供新的思路。This study aimed to explore the pathological changes of α-synuclein （α-syn） in pancreas by injecting small dose ofparaquat into cynomologus monkeys. 12 healthy male cynomolgus monkeys （8-10 years old）, provided by the Wincon Thera Cells Biotechnologies Co. Ltd., were randomly divided into three groups： experimental group A, group B and control group C, with 4 monkeys in each group. Paraquat were injected into the cynomologus monkeys in experimental groups, after which the monkeys in group A died of euthanasia after 5 month, and those in group B died after 2 month （2 monkeys were killed in control group in every stage）. Immunohistochemical method and Western blotting analyses were applied to detect the expression feature and levels of α-syn in pancreas of cynomolgus monkeys. The result showed that α-syn immunoreactive substances were mainly observed in cytoplasmic of islet and there were differential expression of α-syn in different groups. The expression level of α-syn was highest in the experimental group A 5 months after paraquat-injecting, while the control group was at the lowest level. Our research indicated that α-syn expression in pancreas of cynomologus monkeys were up-regulated by intraperitoneal injection of small dose of paraquat, and paraquat was one of the key factors to affect the different expressions of α-syn. It provided new ideas for disease-model making, early diagnosis and intervention.

关 键 词：食蟹猴 百草枯 胰腺 胰岛 Α-突触核蛋白 

分 类 号：R114[医药卫生—卫生毒理学]