灌喂氧化鱼油对黄颡鱼胃肠道黏膜胆固醇和胆汁酸合成途径基因差异表达的影响  

Effects of Oxidized-fish-oil-feed on Genetic Differential Expression of Cholesterol and Bile Acid Synthetic Pathways in Gastrointestinal Mucosa of Yellow Catfish(Pelteobagrus fulvidraco)

作　　者：高敏敏[1] 叶元土[1] 吴萍[1] 蔡春芳[1] 林秀秀[1] 吴代武 何杰[1] 张宝彤 萧培珍 

机构地区：[1]苏州大学基础医学与生物科学学院江苏省水产动物营养重点试验室,苏州215123 [2]北京营养源研究所水产动物系统营养研究开放试验室,北京100000

出　　处：《基因组学与应用生物学》2017年第5期1912-1925,共14页Genomics and Applied Biology

基　　金：国家自然科学基金(K113409511)资助

摘　　要：分别提取灌喂氧化鱼油以及鱼油的黄颡鱼胃肠道肠道黏膜的总RNA。将氧化鱼油组、鱼油组总RNA等量混合后,采用RNA-Seq测序,用Trinity进行de novo拼接、组装,对单一基因进行功能注释;再将氧化鱼油组、鱼油组总RNA分别测序、注释后进行基因表达量分析,并计算氧化鱼油组对鱼油组单一基因的差异表达定量分析,以log_2(OFH/FH)值代表氧化鱼油组相对于鱼油组基因的差异表达量。基因差异表达显示,氧化鱼油导致黄颡鱼胃肠道黏膜组织中胆固醇、胆汁酸生物合成代谢途径的酶以及涉及胆固醇和胆汁酸吸收转运蛋白基因差异表达。黄颡鱼胃肠道黏膜中以乙酰辅酶A为原料的胆固醇生物合成途径关键酶基因差异表达显著下调,从细胞外吸收转运胆固醇的主要蛋白基因差异表达下调,显示灌喂氧化鱼油导致黄颡鱼胃肠道黏膜胆固醇合成能力下降、从其他组织吸收胆固醇的能力下降,血清胆固醇含量下降4.06%。灌喂氧化鱼油后,黄颡鱼胃肠道黏膜以胆固醇为原料的初级胆汁酸合成代谢的关键酶差异表达显著上调,而胆汁酸转运到细胞外和转运到肠腔的载体蛋白基因差异表达下调,肠道再吸收胆汁酸的转运载体蛋白基因差异表达下调及肝细胞从血液吸收转运的载体蛋白基因差异表达上调,而血清胆汁酸含量下降了20.00%。结果表明,胆汁酸的肠肝循环发生障碍,肠腔和血清胆汁酸含量下降、胆汁酸在细胞出现淤积的趋势。In this study, we extracted total RNA from gastrointestinal mucosa of yellow catfish which were fed separately with oxidized fish oil and fish oil. After respectively mixing the total RNA of the oxidized fish oil group and the fish oil group, we applied RNA-Seq de novo with Trinity technology to annotate functions of single gene and analyzed genetic expression after sequencing and annotating total RNA of both groups. Then we carried out quantitative analysis on genetic differential expression of single gene in the oxidized fish oil group to the fish oil group, with log2 （OFH/FH） representing the expression differences of the genes in the oxidized fish oil group versus to the fish oil group. The results of genetic differential expression showed that oxidized fish oil led to genetic differential expression of the enzymes of cholesterol and bile acid biosynthetic pathway, as well as cholesterol and bile acid absorption transporter in the gastrointestinal tract mucosa tissue of the yellow catfish. The differential expression of key enzymes which were related with cholesterol biosynthesis pathway and used acetyl coenzyme A as raw material were significantly reduced, and genetic differential expression of the major protein absorbing and transporting cholesterol from extracellular declined as well. The results indicated that feeding oxidized fish oil decreased the ability of synthesizing cholesterol in gastrointestinal tract mucosa and the ability of absorbing cholesterol from other tissues. And the content of serum cholesterol decreased by 4.06%. After feeding oxidized fish oil, the genetic differential expression of key enzymes that catalyzed the synthetic metabolism and turned cholesterol into primary bile acid in gastric-intestinal mucosa of catfish was remarkable up-regulated, while that of carrier protein that transfer bile acid outside to cells and into intestinal cavities was down-regulated. Besides, the differential expression of carrier protein in the process of intestinal bile acid reabsorbing was down-re

关 键 词：氧化鱼油 黏膜 胆固醇 胆汁酸 黄颡鱼 

分 类 号：S965.199[农业科学—水产养殖]