仿刺参(Apostichopus japonicas)miRNA研究进展  

Research Progress of MiRNA in Sea Cucumber(Apostichopus japonicus)

作　　者：孔令明[1] 陆慧仙 刘娇丽 何瑞琳 岳墨林 苏心玫 

机构地区：[1]山东大学(威海),威海264209

出　　处：《基因组学与应用生物学》2017年第5期1946-1952,共7页Genomics and Applied Biology

基　　金：山东大学科技创新基金项目(项目编号:A16028)资助

摘　　要：MicroRNA (miRNA)是一类普遍存在于长约 22 nt 的非编码小分子 RNA，它通过与靶基因 mRNA特定位点的结合，实现靶基因在转录时的剪切降解，或下调靶基因在转录后的翻译水平。miRNA 在细胞分化、细胞增殖、细胞调亡和新陈代谢等生命活动中发挥重要调控功能，是当前生物学研究领域的一大热点。仿刺参(Apostichopus japonicus)具有很高的营养价值和医药价值，是我国重要的水产养殖经济品种之一。目前，对仿刺参 miRNA 的研究取得了一些进展，本研究就 miRNA 的研究方法与技术，miRNA 参与仿刺参的生长、夏眠、免疫和疾病等过程的研究进展进行综述，并对其 miRNA 研究前景进行展望，以期为今后开展相关研究提供参考。MicroRNAs （miRNAs） are evolutionarily conserved small noncoding RNAs with approximately 22 nucleotides, existing widely in creatures, which combines with the specific sites ofmRNA of target gene to achieve the shear degradation of the target gene at the time of transcription or the down-regulation of translational level of target gene after transcription. The current studies has indicated that miRNAs play critical regulatory roles in a vast range of biological processes including cellular differentiation, proliferation, apoptosis and metabolism, which has become a hot topic in biological research. Sea cucumber （Apostichopus japonicus）, famous for its superior nutritive value and supposed medicinal properties, is one of the most important aquaculture species in China. At present, significant advances have been made in the discovery of novel miRNAs of sea cucumber. This review summarized the research methods and techniques ofmiRNA, reviewed the regulatory function ofmiRNA in the growth, aestivation, immunity and skin ulcer syndrome ofApostiehopus japonieus, discussed the prospects of miRNA research in the hope of providing reference for furture research.

关 键 词：MICRO RNA 仿刺参 生长 夏眠 免疫 腐皮综合症 

分 类 号：S917.4[农业科学—水产科学]