保护酶SOD在木薯种质抗螨中的功能初步分析  

Preliminary Analysis of the Function of Protective Enzyme SOD in Cassava Resistance to Mite

作　　者：梁晓[1] 卢芙萍[1] 卢辉[1] 伍春玲[1] 田桂子 陈青[1] 朱俊洪[2] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所农业部热带作物有害生物综合治理重点实验室海南省热带作物病虫害生物防治工程技术研究中心海南省热带农业有害生物监测与控制重点实验室,海口571101 [2]海南大学环境与植物保护学院,海口570228

出　　处：《基因组学与应用生物学》2017年第5期2055-2060,共6页Genomics and Applied Biology

基　　金：现代农业产业技术体系建设专项资金(No.CARS-12-hncq)资助

摘　　要：目前尚不了解保护酶SOD(superoxide dismutase)在木薯种质抗螨中的作用。为证实SOD的抗螨功能,本研究通过定量PCR和酶学试验手段分析了朱砂叶螨取食抗、感螨木薯种质后,SOD的基因表达量和酶活分别在木薯和朱砂叶螨中的变化情况。结果表明,一方面,朱砂叶螨取食1 d和8 d后,感螨木薯种质BRA900体内MeCu/ZnSOD的表达量和SOD总酶活仅分别较为害前提高1.15倍、1.16倍和1.17倍、1.14倍,而在抗螨木薯种质C1115体内则分别较为害前提高2.47倍、2.86倍和2.18倍、2.42倍,显著高于感螨木薯水平。另一方面,朱砂叶螨取食感螨种质BRA900 1 d和8 d后,保护酶TcSOD的基因表达量和SOD酶活分别是为害前的0.99倍、1.09倍和1.12、1.05倍,而取食抗螨木薯种质C1115 1 d和8 d后分别降低至为害前的0.51倍、0.46倍和0.49倍、0.56倍,显著低于取食感螨木薯水平。本研究初步证实了保护酶SOD在木薯种质抗螨中的功能,为将其作为潜在基因资源应用于抗螨木薯种质创制提供理论基础。So far, there has been a lack of knowledge about the function ofsuperoxide dismutase （SOD） in cassava resistance to mite. In order to validate this function, we used quantitative Real-time PCR （qPCR） and enzymatic assay to analyze the changes of gene expression and enzyme activities of SOD in cassava and carmine spider mite （Tetranychus cinnabarinus） after carmine spider mite was fed by mite-resistant and mite-susceptible cassava cultivars. The results showed that, on the one hand, compared with those in the same leaves before damaged, the the transcriptions of MeCu/ZnSOD and total activities of SOD in mite-susceptible cassava cultivar BRA900 damaged by carmine spider mite （T. cinnabarinus） for 1 d and 8 d only increased by 1.15 times, 1.16 times and 1.17 times, 1.14 times, respectively. However, the corresponding values in 1 d and 8 d damaged leaves of C1115 increased by 2.47 times, 2.86 times and 2.18 times, 2.42 times, respectively, which were significantly higher than those in mite-susceptible cassava. On the other hand, compared with those before feeding, the transcriptions of TcSOD and activities of SOD in carmine spider mite fed by BRA900 for 1 d and 8 d increased by 0.99 times, 1.09 times and 1.12 times, 1.05 times, respectively, while the corresponding values in carmine spider mite fed by C1115 decreased by 0.51 times, 0.46 times and 0.49 times, 0.56 times, respectively. This study preliminarily validated the function of SOD in cassava resistance to mite, and provided theoretical basis for applying it as a potential genetic resource to the germplasm of anti-mite cassava.

关 键 词：SOD 朱砂叶螨 木薯种质 抗螨 

分 类 号：S435.33[农业科学—农业昆虫与害虫防治]