利用红外光谱和高效液相色谱比较分析不同产地红根草丹参酮ⅡA含量  

Comparison Analysis of Tanshinone Ⅱ A Content in Salvia priontis from Different Regions by Utilizing FTIR and HPLC

作　　者：梁惠凌 孔德鑫 王满莲 邹蓉 黄庶识[2] 文香英[3,4] 

机构地区：[1]广西植物研究所广西植物功能物质研究与利用重点实验室,桂林514006 [2]广西科学院生物物理实验室,南宁530003 [3]中国科学院华南植物园,广州510650 [4]国际植物保护联盟(BGCI),广州510650

出　　处：《基因组学与应用生物学》2017年第5期2097-2101,共5页Genomics and Applied Biology

基　　金：国际植物园保护联盟项目(BGCI 30412);广西重大专项计划项目(桂科重1298001-1-3);桂林市科技合作与交流项目(20140122-4);桂林市科技成果转化与推广项目(20140124-2);桂林市科技攻关项目(2016010305-2)共同资助

摘　　要：红根草是广西道地药材,丹参酮ⅡA是红根草的主要活性作用成分之一,因此,筛选出丹参酮ⅡA含量高的优良种质,对解决红根草资源紧缺问题及提高其药材质量均有重要作用。本研究利用傅里叶红外光谱技术对不同产地红根草化学成分的红外吸收特征峰进行了测定,利用HPLC技术不同产地红根草中丹参酮ⅡA含量,同时,借助化学计量学方法比较分析了不同产地样品的红外光谱吸收峰与丹参酮ⅡA相关性。结果表明,不同产地红根草化学成分组成比较相似,但化学成分的含量却有较大差异。多元对数相关分析技术显示,1 734 cm^(-1)、1 632 cm^(-1)、1 548 cm^(-1)、1 515 cm^(-1)、830 cm^(-1)及789 cm^(-1)等处附近的吸收强度与实际测定不同产地红根草丹参酮ⅡA含量均有密切关系。两种方法均一致显示,丹参酮ⅡA含量以江西南丰类型最高,其次是广西钟山类型,其中以江西奉新类型含量最低。Salvia prionti is a famous region drug in Guangxi, and tanshinone ⅡA is one of the main effective components in S. priontis. Therefore, screening out S. priontis species that contain high content of tanshinone ⅡA will play an important role in solving the shortage of the resources of S. priontis and improving its raw materials quality. In present research, the infrared absorption peak of chemical composition in S. priontis from different origins was measured by fourier transform infrared spectrum （FTIR）, the content of tanshinone I] A in S. priontis from different regions were detected by HPLC, whilst, the correlation between FTIR peak of S. priontis and the changes of tanshinone in different origins were compared by using the methods of chemometric. The results indicated that the chemical compositions of S. priontis of different origins were similar, but the contents had significant differences. Multivariate logarithmic correlation analysis showed that the variation of the absorption peaks around 1 734 cm-1, 1 632 cm-1, 1 548 cm-1, 1 515 cm-1, 830 cm-1, 789 cm-1 were closely related with the changes of the measured value of tanshinone ⅡA in S. priontis from different origins. In conclusion, two kinds of methods consistently revealed that the highest content of tanshinone Ⅱ A was in S. priontis from Nanfeng, Jiangxi, the second was the origin from Zhongshan, Guangxi, and the lowest content was in the type from Fengxin, Jiangxi.

关 键 词：红外光谱图 丹参酮ⅡA 红根草 

分 类 号：R284.1[医药卫生—中药学]