新合成查尔酮类衍生物对大鼠同种异体移植术后急性排斥反应阶段CD4^＋／CD8^＋T细胞比值的影响  

Influence of the new synthesis of chalcones derivative L6H21 on CD4^＋/CD8^＋T cell ratio at acute rejection stage following rat limb allograft

作　　者：余方正 温新[1] 丁伟力 朱君毅[1] 杜胜虎 张怀保[1] 卓高豹[1] 王健[1] 

机构地区：[1]温州医科大学附属第一医院手外科周围神经外科,浙江325000 [2]玉环县人民医院骨科

出　　处：《中华手外科杂志》2017年第3期217-220,共4页Chinese Journal of Hand Surgery

基　　金：浙江省自然科学基金项目（LY13H060006）;温州市科技局项目（Y20140574）

摘　　要：目的探索新合成查尔酮类衍生物L6H21对大鼠同种异体肢体移植急性排斥反应阶段CD4^＋／CD8^＋T细胞比值的影响。方法取36只健康雄胜SD大鼠的后肢作为供体，取72只健康雄性Wistar大鼠作为受体，建立大鼠后肢异体移植模型。随机分为三组：对照组（A组），环孢素A组（B组），查尔酮类衍生物L6H21组（C组）。于移植术后1、3、5、7d观察各组大鼠移植后肢的外观变化、排斥反应出现时间。流式细胞仪分选CD4^＋、CD8^＋T细胞，观察急性排斥反应阶段CD4^＋／CD8^＋T细胞比值的变化。结果术后A组大鼠平均存活时间为（6．00±0．67）d，B组为f23．62±1．21）d，C组为（25．34±1．70）d。术后第1天，所有组中均未发现CD4^＋／CD8^＋T细胞比值差异。术后第3天，A组CD4^＋／CD8^＋T细胞比值明显升高，与其他两组相比差异有统计学意义（P〈0．05），B组和C组比较差异无统计学意义（P〉0．05）。术后第5天，虽然CD4^＋／CD8^＋T细胞比值在所有组中均降低，但B、C组降低更为显著，且C组较B组更为明显，差异均具有统计学意义（P〈0．05）。结论新合成查尔酮类衍生物L6H21能抑制大鼠同种异体肢体移植术后急性排斥反应阶段CD4^＋／CD8^＋T细胞比值，并能够延长大鼠移植肢体的存活时间。Objective To explore the imfluence of the new synthesis of chalcones derivative L6H21 on CD4^＋/CD8^＋T cell ratio at acute rejection stage following rat limb allograft. Methods The hind limbs of 36 healthy male SD rats were served as donors, and 72 healthy male Wistar rats were used as recipients to establish the rat model of hind limb allograft. All the rat models were randomly divided into three groups according to the different postoperative immunosuppressive regimes： 0.9% NaCI control group （group A）, cyclosporine A group （group B）, chalcone derivative L6H21 group （group C）. At 1, 3, 5 and 7 days after the allograft transplantation, appearance changes and rejection occurrence time of the transplanted limbs from each group were observed. CD4^＋ and CD8^＋T cells were sorted by flow cytometry in order to figure out the changes of CD4^＋/CD8^＋T cell ratio. Results The average survival time was （6.00±0.67） d for group A, （23.62±1.21） d for group B and （25.34±1.70） d for group C. At 1 day after the surgery, no CD4^＋/CD8^＋T cell ratio difference was found among all the groups. At 3 days postoperatively, CD4^＋/CD8^＋T cell ratio was obviously increased in group A. Compared with the other two groups, the difference was statistically significant （P〈0.05） and was not between group B and C （P〉0.05） . After 5 days postoperatively, CD4^＋/CD8^＋T cell ratio was decreased in all the groups, and group B and C reduced more significantly （P〈0.05）. Conclusion The new synthesis of chalcones derivative L6H21 can reduce CD4^＋/CD8^＋T cell ratio at acute rejection stage following rat limb allograft, and prolong the survival time of rat limb.

关 键 词：移植 同种 动物实验 查尔酮衍生物E6H21 CD4+/CD8+T细胞比值 

分 类 号：R658[医药卫生—外科学]