Apelin-13体外促进猴视网膜血管内皮细胞增殖和迁移及管腔形成  

Research on the promoting role of apelin-13 in proliferation,migration and capillary-like tube formation of RF/6A cells

作　　者：谢坤鹏 刘平[2] 王新[2] 杜军辉 

机构地区：[1]河南省立眼科医院,中国河南省郑州市450003 [2]郑州人民医院眼科,中国河南省郑州市450003 [3]中国陕西省西安市第九医院眼科,710054

出　　处：《国际眼科杂志》2017年第6期1042-1046,共5页International Eye Science

基　　金：陕西省自然科学基础研究计划--面上项目(No.2013020)~~

摘　　要：目的:通过观察apelin-13对体外培养的猴脉络膜/视网膜内皮细胞(RF/6A)增殖、迁移和毛细血管样管腔形成的影响,探讨apelin-13是否对视网膜新生血管的发生具有促进作用。方法:取生长状况良好的RF/6A细胞,分为对照组、低剂量组(0.1μmol/L apelin-13)和高剂量组(1μmol/L apelin-13)。培养细胞24h后采用MTT法检测细胞增殖,细胞划痕法检测细胞迁移,基质胶法检测管腔形成。结果:不同浓度apelin-13作用24h的细胞增殖强于对照组,差异有统计学意义(P<0.05),不同浓度apelin-13组RF/6A细胞24h的迁移距离大于对照组,差异有统计学意义(P<0.05),不同浓度apelin-13组毛细血管样管腔形成数明显多于对照组,差异有统计学意义(P<0.05),且细胞增殖、迁移和管腔形成均随着apelin-13浓度的升高而增加。结论:Apelin-13能够明显促进RF/6A细胞的血管生成过程,提示apelin-13是一种促视网膜新生血管形成的重要因子。AIM：To investigate the effects of apelin- 13 on proliferation, migration and capillary-like tube formation of a monkey choroid / retinal endothelial cell line, RF/ 6A, to clarify whether apelin-13 could promote retinal angiogenesis in vitro. METHODS： RF/6A cells in good conditions were administrated with DMSO （the control group）, apelin-13 at 0. 1μ mol/L （ low dose group） or apelin-13 at 1μ mol/L （high dose group）. Cell proliferation, migration and capillary-like tube formation were detected by using the MTT assay, scratch assay and matrigel assay, respectively, at 24h after plating the cells. ~ RESULTS： Cell proliferation was promoted in both low and high dose apelin-13 groups compared to the control cells （P〈0.05） ; the cell migration distance of both apelin-13 groups was significantly greater than that of the control group （P〈0.05） and the number of capillary-like tube structures of both apelin - 13 groups was significantly larger than that of the control cells （P〈 0.05）. In addition, cell proliferation, migration and tube formation increased as the concentration of apelin-13 increased. CONCLUSION： Apelin-13 could obviously promote the angiogenesis capacity of RF/6A cells, suggesting that apelin-13 was an important pro-angiogenic factor in retinal endothelial cells.

关 键 词：APELIN APELIN-13 血管生成 视网膜新生血管 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]