载血管内皮生长因子层层自组装提高异种去细胞血管生物相容性  

Layer-by-layer assembly delivered VEGF enhances biocompatibility of decellularized scaffolds

作　　者：谭琦[1] 李娜[2] 陶运明[3] 唐浩[3] 吴忠仕[3] 

机构地区：[1]山东大学附属省立医院心外科,山东济南250014 [2]山东大学附属省立医院妇科,山东济南250014 [3]中南大学湘雅二医院心外科,湖南长沙410011

出　　处：《山东大学学报（医学版）》2017年第5期23-30,共8页Journal of Shandong University:Health Sciences

基　　金：山东省医药卫生科技发展计划(2013WS0115)

摘　　要：目的探讨血管内皮生长因子(VEGF)结合肝素/壳聚糖层层自组装技术修饰异种去细胞血管,提高其生物相容性。方法采用肝素/壳聚糖在异种去细胞血管表面层层自组装,以1-乙基-(3-二甲基氨基丙基)碳酰二亚胺(EDC)进行化学交联,并负载VEGF。采用扫描电镜检测血管表面微结构,甲苯胺蓝法定量分析肝素结合量及缓释性能,采用ELISA法定量分析VEGF结合量及缓释性能,抗凝血活性和血小板黏附试验检测材料血液相容性,采用细胞增殖试验评价材料的细胞增殖能力。结果肝素/壳聚糖层层自组装在异种去细胞血管表面形成纳米颗粒样结构,EDC化学交联显著降低肝素及VEGF释放速率,并提高血管的血液相容性。层层自组装/化学交联并负载VEGF促进内皮细胞增殖。结论载VEGF层层自组装技术修饰异种去细胞血管,能够改善其生物相容性,促进内皮细胞增殖。Objective Heparin/chitosan layer-by-layer （LBL） assembly delivered vascular endothelial growth factor （VEGF） on decellularized scaffolds （DS） was designed for better biocompatibility. Methods LBL was crosslinked by 1-ethyl-（ 3- dimethylamino propyl） carbonyl diimine （EDC）, and VEGF was delivered on decellularized bovine jug- ular vein to produce different scaffolds. The microstructure surface of different scaffolds was observed with scanning electron microscopy （SEM）. The release performance of heparin and VEGF was determined with elution test. The blood compatibility was tested with anticoagulant activity and platelet adhesion test. The endothelialization on scaffolds was analyzed with cell proliferation. Results Heparin/chitosan multilayer films formed the surface coating by nanopar- ticies. EDC improved the stability of multilayer films. The release rate of heparin and VEGF was lower in LBL/EDC-DS group than in LBL-DS group, and the anticoagulant activity and platelet adhesion were better in LBL/EDC-DS group than in LBL-DS group. Live cell staining and MTT test showed that LBL/EDC delivered VEGF significantly in- creased proliferation of endothelial cells on scaffolds. Conclusion Heparin/chitosan layer-by-layer technology deliv- ered VEGF can improve biocompatibility and accelerate endothelialization on decellularized scaffolds.

关 键 词：层层自组装 异种去细胞血管 化学交联 血管内皮细胞生长因子 生物相容性 

分 类 号：R339[医药卫生—人体生理学]